皖南蝮蛇毒蛋白C激活物的急性毒性作用的研究  

Acute toxicity of protein C activator from venom of Agkistrodon halys in rats and mice

作　　者：段婷[1] 张根葆[1,2] 周兵[1] 

机构地区：[1]皖南医学院病理生理学教研室 [2]皖南医学院蛇毒研究室

出　　处：《中国临床药理学与治疗学》2012年第1期20-24,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：安徽省教育厅自然科学基金重点资助项目(KJ2011A266)

摘　　要：目的:探讨蛇毒蛋白C激活物(protein Cactivator,PCA)对大、小鼠的急性毒理反应。方法:大鼠采用上下剂量增减法,小鼠采用半数致死量法,观察实验动物症状、体征、死亡时间和累积死亡率。结果:PCA尾静脉注射大鼠(剂量17.5～175mg/kg)、小鼠(剂量为6.25～11mg/kg)的中毒表现为不同程度的口鼻泡沫状出血、呼吸困难、心率加快、走路不稳、蜷缩、昏厥性抽搐;大、小鼠尾静脉注射PCA的LD50及其95%可信限分别为29.57(17.5～55)mg/kg和8.05(7.55～8.54)mg/kg;大体解剖可见双肺有片块状出血,病理镜检显示出血主要累及肺泡、支气管等。其他器官未见明显异常。结论:皖南蝮蛇毒PCA的毒性主要是抗凝过度导致的出血,肺出血引起的急性呼吸衰竭是实验动物死亡主要原因。ABSTRACT AIM： To investigate the acute toxicity of protein C activator from venom of Agkis- trodon halys in rats and mice. METHODS： Rats using the up-and-down procedure and mice using median lethal dose; the acute toxical symptoms, signs, death time and accumulated death rate were observed. RESULTS：PCA tail intravenous of rats （dose of 17.5-175 mg/kg）, mice （dose of 6.25- 11 mg/kg） poisoning in different de gree of mouth and nose foam bleeding, dyspnea, heart rate, unstable walking, crouching, con- secutive convulsion. The LDs0 and its 95%confi- dent limit of PCA to rats and mice were 29.57 （17.5-55） mg/kg and 8.05 （7.55-8.54） rag/kg respectively. A massive hemorrhage was noted in double lung, and pathological examination shows hemorrhage mainly involved in the alveoli, bronchi, etc. Other organs showed no obvious abnormality. CONCLUSION： The main toxicity of PCA from venom of Agkistrodon halys was hemorrhage induced by anticoagulation. In addition, the acute respiratory failure induced by pneumorrhagia was the major cause for animal death.

关 键 词：蛋白C激活物 急性毒性 半数致死量 

分 类 号：R965.2[医药卫生—药理学] R996.3[医药卫生—药学]