磷酸三钙涂层镁合金材料的细胞相容性研究  

Study on Cell Compatibility of Magnesium Alloy Covered with Tricalcium Phosphate

作　　者：付玉平[1] 郭磊[1] 管俊林[1] 赵杰[1] 赵延刚[1] 谭丽丽[2] 杨柯[2] 

机构地区：[1]中国医科大学附属第一医院,沈阳110001 [2]中国科学院金属研究所,沈阳110016

出　　处：《中国生物工程杂志》2012年第2期45-49,共5页China Biotechnology

基　　金：国家自然科学基金(30872724;81071460);辽宁省科技攻关层次计划(2008225009;2009225010-3)资助项目

摘　　要：目的:制备磷酸三钙(β-TCP)涂层镁合金材料,评价材料表面的特性及体外的细胞生物适应性。方法电化学法制备β-TCP涂层镁合金材料(β-TCP-Mg-AI-Zn),观测金属材料表面微观结构特性和能谱分析,小鼠颅骨源成骨细胞与材料直接接触培养,荧光染色观察材料表面细胞生长状况,检测成骨细胞增殖和碱性磷酸酶(ALP)活性。结果β-TCP涂层Mg-AI-Zn材料表面呈多孔状,材料表面含有镁、钙和磷等元素;成骨细胞与材料直接接触培养24 h及48 h后,材料表面有大量的成骨细胞粘附、伸展、汇合;与Mg-AI-Zn材料比较,β-TCP-Mg-AI-Zn材料明显地促进细胞增殖、显著地增加成骨细胞中ALP活性(P<0.05)。结论β-TCP涂层改善了Mg-AI-Zn镁合金材料表面特性及体外的细胞相容性,有望成为新一代可降解医用金属材料。The magnesium electrosynthesized and evaluated its alloy surface （Mg-AI-Zn） covered with tricalcium characteristics and biocompatibility. The phosphate （[β--TCP） was β--TCP-Mg-AI-Zn has beenprepared by electrochemistry, whereas the surface structure and spectrum analysis of material were measured. The osteoblasts from mice skull were cultured with magnesium alloys in vitro, and the cell proliferation and alkaline phosphatase （ALP）were detected with methyl thiazolyl tetrazolium and fluorescein isothiocyanate. The results show that the 15-TCP-Mg-AI-Zn surface was porosity and high content of magnesium, calcium and phosphorus elements. The cell proliferation and the activity of osteoblasts were increased after cultured 24 h or 48 h with the β-TCP-Mg-AI-Zn （P 〈 0.05 ）. Therefore, covering with β-TCP has improved surface properties and cell compatibility in vitro. The magnesium alloy covered with β-TCP would be a new biomedical material.

关 键 词：镁合金磷酸三钙 涂层成骨细胞细胞相容性 

分 类 号：Q819[生物学—生物工程]