枳椇子对非酒精性脂肪肝大鼠血清TNF-α、IL-6、IL-8水平的影响  

EFFECTS OF HOVENIA DULCIS THUNB ON SERUM TNF - α,IL - 6 AND IL - 8 LEVELS IN RATS WITH NON - ALCOHOLIC FATTY LIVER

作　　者：徐晶莹[1] 朱肖鸿[2] 胡洁[2] 倪桂宝[2] 孙明洪[2] 

机构地区：[1]杭州市第六人民医院,杭州310014 [2]浙江中医药大学附属第一医院,杭州310018

出　　处：《中国中医药科技》2012年第1期35-36,38,共3页Chinese Journal of Traditional Medical Science and Technology

摘　　要：目的:观察枳椇子对高脂饮食诱导的非酒精性脂肪肝(NAFLD)大鼠肝组织学变化和血清TNF-α、IL-6、IL-8水平的影响,探讨其防治NAFLD的作用机理。方法:以高脂饮食喂养SD大鼠90天建立非酒精性脂肪肝模型,同时以不同剂量的枳椇子连续干预90天,观察肝组织学的病理改变,放射免疫法测定血清TNF-α、IL-6、IL-8水平。结果:模型组大鼠肝组织脂肪变性、炎症活动度、血清中TNF-α、IL-6、IL-8水平较正常组明显升高;枳椇子高、低剂量组大鼠肝组织炎症程度、血清TNF-α、IL-6、IL-8含量明显降低。结论:TNF-α、IL-6、IL-8参与高脂饮食诱导的大鼠非酒精性脂肪肝的发生;降低血清TNF-α、IL-6、IL-8水平可能是枳椇子防治非酒精性脂肪肝进展的重要机制之一。Objective: To observe the effects of hovenia dulcis thunb on histological changes of liver and serum TNF - α,IL - 6,and IL - 8 levels in rats with non - alcoholic fatty liver,discuss the mechanism. Methods: SD rats were feeding high fat diet for 90 days to build non - alcoholic fatty liver models,different doses hovenia dulcis thumb liquid were given to intervene the formation of non - alcoholic fatty liver for 90 days at the same time. The histological changes of rats’liver were observed by light microscope,serum TNF - α,IL - 6,and IL - 8 levels wre determined by radioimmunoassay. Results: The degree of fatty degeneration and inflammation activity of hepatic tissue in model group was obvious,serum TNF - α,IL - 6,and IL - 8 levels of model rats were significantly higher than those of normal rats; the degree of inflammation activity of hepatic tissue in high and low dose hovenia dulcis thunb groups was obviously improved,serum TNF - α,IL - 6,and IL - 8 levels in the two dose hovenia dulcis thumb groups were significantly reduced. Conclusion: TNF - α,IL - 6,and IL - 8 were related to the occurrence of non - alcoholic fatty liver induced by high fat diet,decrea- sing serum TNF - α,IL - 6,and IL - 8 contents maybe one of the mechanism that hovenia dulcis thumb can effectively prevent non - alcoholic fatty liver.

关 键 词：脂肪肝/中医药疗法 枳椇子/治疗应用 脂肪肝/病理学 大鼠 

分 类 号：R285.5[医药卫生—中药学]