亚低温对颅脑创伤大鼠脑组织中β-分泌酶表达的影响  

The effects of mild hypothermia on expression of β- secretase after traumatic brain injury in rats

作　　者：程世翔[1] 涂悦[1] 孙洪涛[1] 令狐海瑞[1] 张赛[1] 

机构地区：[1]武警部队脑创伤与神经疾病研究所、武警医学院脑系科中心,天津300162

出　　处：《中华神经外科杂志》2012年第2期153-156,共4页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金（30872668）;武警医学院院级重点项目（WYZ201002）

摘　　要：目的研究亚低温对大鼠颅脑创伤（TBI）后β-分泌酶（BACE）的影响。方法应用液压冲击装置制作大鼠颅脑创伤模型，实验分为假手术组、创伤常温组（37℃）和创伤亚低温组（32℃），每组12只大鼠。采用免疫组织化学和免疫荧光法检测BACE表达部位，采用RT-PCR和免疫印迹法研究BACEmRNA和蛋白表达水平。结果与假手术组比较，创伤常温组大鼠脑组织BACE免疫阳性细胞数明显增多，BACEmRNA和蛋白表达上升（P＜0．05）。但是，可以看出BACE在给予亚低温处理后的免疫阳性细胞数量以及蛋白水平显著低于常温处理组（P＜0．05），与假手术组比较差异无统计学意义。结论亚低温可以通过降低颅脑创伤后BACE表达水平来减轻继发性脑损伤程度，起到神经保护作用。Objective To investigate the effects of mild hypothermia treatment on the expression of β -secretase （BACE） after traumatic brain injury （TBI） in rat model. Methods Adult male Sprague - Dawley rats were randomly divided into three groups ： sham - operated group, TBI with normothermia group （37℃）, and TBI with hypothermia group （32℃）. The traumatic brain injury rat model was established with a fluid percussion device. Mild hypothermia intervention for 6 h immediately initiated after TBI in hypothermia group. At 24 h after TBI, rats were sacrificed and the brain hippocampus tissues were prepared for hematoxylin and eosin staining. The immunohistochemistry and immunofluorescence were used for the location of BACE. Gene transcription and protein expression of BACE were examined by reverse transcription -polymerase chain reaction （RT -PCR） and Western blotting, respectively. Results Normothermia group showed increased BACE higher than that of sham - operated group （ P 〈0. 05 ） . However, hypothemlia group showed significantly lower expression of BACE compared with normothermia group （ P〈0.05）, which showed no significant difference compared to the sham - operated group. Conclusions Mild hypothermia treatment, which plays a neuroprotective role, might decrease the level of BACE induced by traumatic brain injury, resulting in secondary brain injury.

关 键 词：外伤性脑损伤 亚低温 Β-分泌酶 

分 类 号：R651.15[医药卫生—外科学]