水稻紫色柱头突变体ps-5的遗传分析和分子定位  

Genetic Analysis and Molecular Mapping of a Purple-stigma Mutant(ps-5) in Rice

作　　者：李万昌[1] 王俊伟[1] 余娇娇[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2012年第1期121-124,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省科技攻关项目(102102310318);河南省教育厅项目(2010A210021)

摘　　要：在水稻品种Nipponbare中发现一个紫色柱头突变体ps-5,整个生育期最显著的特点就是柱头紫色且子房较大.遗传分析表明该基因受1对隐性核基因控制,暂命名为ps.利用极端个体定位法把ps-5精细定位于第8染色体SSR标记LR41和LR43之间,ps-5基因距它们的遗传距离均为0.12cM,并与LR413共分离,LR41和LR43两标记间的物理距离约为125kb,为该基因的分子标记辅助选择和图位克隆奠定了基础.A rice（Oryza sativa L.） mutant with a purple-stigma,designated as ps-5,is found from a japonica rice variety Nipponbare.The most distinctive trait of this mutant is that its ovary is bigger than that in wild-type rice.Genetic analysis show that this phenotype of ps-5 is controlled by a single recessive nuclear gene,temporarily designated as ps-5.By means of molecular marker technique and high-resolution linkage analysis,the ps-5 gene is restricted to two SSR markers LR41 and LRM43 on chromosome 8.The genetic distance is 0.12 cM to each marker respectively,and co-segregated with LR413.The physical distance between LR41 and LRM43 is about 125 kb.This is the basis for marker-assisted selection（MAS） and map-based coloning this ps-5 gene.

关 键 词：水稻 紫色柱头 遗传分析 基因定位 

分 类 号：S511.4[农业科学—作物学]