SUMO4、NF-κB、IκB在2型糖尿病大鼠肾脏中的表达及意义(英文)  

Expressions of SUMO4,NF-κB and IκB in kidney of rats with type 2 diabetes mellitus and its significance

作　　者：陈思娇[1] 王大南[2] 徐锦春[1] 李红燕[1] 刘芙蓉[2] 宋今丹[2] 

机构地区：[1]中国医科大学附属第一医院干部医疗工作部老年病教研室,辽宁沈阳110001 [2]中国医科大学医学分子生物学研究所卫生部细胞生物学重点实验室

出　　处：《心血管康复医学杂志》2012年第1期1-4,F0002,共5页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：辽宁省科学技术项目(2011225052-2011020216-428)~~

摘　　要：目的:探讨2型糖尿病大鼠肾脏组织小泛素相关修饰蛋白4(SUMO4)、核转录因子(NF)-κB、NF-κB的抑制因子(IκB)的表达及意义。方法:取10只40周龄的无特定病原体(SPF)级雄性自发性糖尿病(GK)大鼠,10只40周龄的SPF级雄性Wistar大鼠,通过HE染色法观察肾组织病变、免疫组化法观察肾组织的SUMO4与IκB、SUMO4与NF-κB表达情况。结果:GK大鼠的肾小球毛细血管球肥大,基底膜轻度增厚,肾小球系膜细胞增生、肥大,肾小管上皮细胞肥大。与正常Wistar大鼠比较,GK大鼠的肾脏NF-κB[(0.232±0.034)比(0.634±0.058)]、IκB[(0.242±0.027)比(0.712±0.078)]及SUMO4[(0.160±0.031)比(0.545±0.045)]的表达水平均明显升高(P均<0.01)。结论:NF-κB、IκB及SUMO4在GK大鼠肾脏组织表达增多。从而推断在2型糖尿病大鼠肾脏SUMO可能抑制NF-κB转录活性,SUMO化可能成为治疗糖尿病肾脏微血管病变的一个新靶点。Objective：To study expressions of small ubiquitin-related modifier protein（SUMO）4（SUMO4）,nuclear factor（NF）-κB and inhibitory factor of NF-κB（IκB） in kidneys of rats with type 2 diabetes mellitus（T2DM）.Methods：A total of ten 40-week-old male Goto-Kakizaki（GK） rats（with spontaneous diabetes mellitus）of specific-pathogen free（SPF） grade,and ten 40-week-old male Wistar rats of SPF grade were selected.The lesion of renal tissue was observed by hematoxylin eosin（HE） staining.Experssions of SUMO4,NF-κB and IκB in renal tissue were observed by immunohistochemistry methods.Results：In the GK rats,glomerular capillary ball hypertrophy,basilar membrane slightly thickening;glomerular mesangial cells hyperplasia,hypertrophy and renal tubular epithelial cells hypertrophy were observed.Compared with normal Wistar rats,expression levels of NF-κB [（0.232±0.034） vs.（0.634±0.058）],IκB [（0.242±0.027） vs.（0.712±0.078）]and SUMO4 [（0.160±0.031） vs.（0.545±0.045）]significantly increased in renal tissue of GK rats（P〈0.01 all）.Conclusion：Compared with Wistar rats,expressions of NF-κB,IκB and SUMO4 significantly increase in renal tissue of GK rats,suggesting that SUMO inhibiting transcriptional activity of NF-κB may exist in kidneys of T2DM rats.Therefore,sumoylation may be a new therapeutic target for inhibit renal microvascular lesion of diabetic disease.

关 键 词：小泛素相关修饰蛋白质类 转录因子 糖尿病 2型 大鼠 

分 类 号：R587.109[医药卫生—内分泌]