羊毛角蛋白的提取工艺及其特性表征  

The Extraction and Characterization of Keratin from Wool

作　　者：付学忠[1] 刘琳[1] 姚菊明[1] 

机构地区：[1]浙江理工大学先进纺织材料与制备技术教育部重点实验室,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2012年第2期160-163,共4页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：教育部新世纪人才支持计划(NCET070763)

摘　　要：采用化学还原法从羊毛中提取角蛋白,制备角蛋白膜,并采用红外、电泳等测试方法对提取的角蛋白进行表征,同时探讨在透析液中加入少量十二烷基磺酸钠对角蛋白提取过程的影响。结果表明:提取角蛋白的分子量集中在50～70kD;角蛋白在溶解过程中并没发生肽键的断裂,在透析时部分断裂的二硫键再次被氧化;透析液中加入十二烷基磺酸钠,可溶性角蛋白的产率由56%提高到78%,且十二烷基磺酸钠的加入对所提取角蛋白的分子量和内部结构没有明显影响。Keratin is extracted from the wool by the chemical reduction method to prepare the keratin membrane. The keratin solution and membranes are characterized by FTIR, SDS/PAGE. The influence of the addition of SI）S in the dialysate on the keratin extraction is also investigated in the study. The results show that the molecular weight of the keratin derived from the wool generally distributes from 50 to 70 KD No peptide bonds fracture in the dissolution process of the keratin, and part of the fractured disulfide bonds are oxidized during the process of dialysis~ The yield of keratin increases from 56% to 78% after the addition of SDS in the dialysate, without causing any effects on the molecular weight and structure of the extracted keratin.

关 键 词：羊毛 角蛋白 十二烷基磺酸钠 产率 分子量 二级结构 

分 类 号：TS102.31[轻工技术与工程—纺织工程]