大肠杆菌O157:H7实验感染动物排菌动态检测  

Detection of the faeces of animals inoculated with E.coli O157:H7

作　　者：孙茂华[1] 孟祥升[2] 夏兴霞[2] 赵攀登[2] 张雪寒[2] 何孔旺[2] 王永山[2] 

机构地区：[1]南京军区联勤部门诊部,江苏南京210002 [2]江苏省农业科学院兽医研究所,江苏南京210014

出　　处：《东南国防医药》2012年第1期5-8,共4页Military Medical Journal of Southeast China

基　　金："十一五"国家科技支撑计划(2007BAD40B01)

摘　　要：目的检验大肠杆菌O157:H7胶体金免疫层析检测试纸条在感染动物排菌中的应用效果。方法将牛和小鼠采用灌胃攻毒方式实验感染大肠杆菌O157:H7,用大肠杆菌O157:H7胶体金免疫层析检测试纸条与细菌鉴别培养基分别培养检测感染动物粪便中的O157:H7的排菌量和持续时间。结果牛在感染大肠杆菌O157:H7后的第2天开始从粪便排菌,第4～6天达到峰值,排菌时间可持续28 d;小鼠在感染O157:H7后的第4小时开始从粪便排菌,第6小时达到峰值,排菌时间可持续15 d。结论大肠杆菌O157:H7胶体金免疫层析检测试纸条与细菌鉴别培养基培养计数的检测结果一致,试纸条更简便、快捷、直观,在1～5 min内可得出检测结果。Objective To investigate the application of the gold immunochromatographic strip for the detection of animals inoculated with E. coli O157 ： H7. Methods The cattle and mice were inoculated intragas- trically with E. coli O157 ：H7, and their faeces were detected by using the gold immunochromatographic strip and counting colony. Results E. coli O157 ： H7 in faeces could be detected on day 2 after inoculation in inoculated cattle by using immunochromatographic strip of E. coli O157 ：H7 and the method of plate culture count with dif- ferential medium of E. coli O157：H7 and the peak levels of O157：H7 in faeces were attained during the forth to sixth day after inoculation and then it dropped down gradually up to day 28 following inoculation. The O157 ：H7 in faeces could be detected on hour 4 after inoculation in inoculated mice by using IC strip of E. coli O157 ：H7 and the method of plate culture count with differential medium of E. coli O157 ： H7 and the peak levels of O157 ： H7 in faeces were attained during the sixth hour after inoculation and then it dropped down gradually up to day 15 following inoculation. Conclusion The gold immunochromatographic strip for the detection of E. coli O157 ： H7 is convenient and rapid for the clinical diagnosis and field investigation of E. coli O157 ： H7 infection or/and contamination.

关 键 词：胶体金免疫层析检测试纸条 肠出血性大肠杆菌O157:H7 牛 鼠 粪便 检测 

分 类 号：R378.2[医药卫生—病原生物学]