RP-HPLC-ELSD法测定野生白花丹药材1～12月份根、茎、叶中β-谷甾醇的含量  

Determination of the Content of β-Sitosterol in Roots,Stems,Leaves of 1-12 Month-old Wild Plumbaginis Zeylanicae by HPLC

作　　者：戴先芝[1,2] 丁玲[2] 黄艳菲[2] 李艳丹[2] 张吉仲[2] 刘圆[2] 

机构地区：[1]阿坝师范高等专科学校,四川汶川623002 [2]西南民族大学民族医药研究院,四川成都610041

出　　处：《云南中医中药杂志》2012年第2期55-56,1,共2页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：四川省杰出青年学术技术带头人后续计划(NO:2011JQ0051);四川省科技支撑计划(NO:2011SZ0233);中央高校基本科研业务费专项资金项目优秀科研团队及重大孵化项目(NO:11NZYTH02);阿坝师专校级重点课题(NO:ASA11-31)

摘　　要：目的:测定野生白花丹药材1～12月份根、茎、叶中β-谷甾醇的含量。方法:采用HPLC-ELSD法测定白花丹中β-谷甾醇的含量。色谱条件为:流动相为纯甲醇;色谱柱为迪马C18柱(250mm×4.6mm,5μm);流速为1.0mL/min;柱温25℃;蒸发光散射检测器检测参数为漂移管温度65℃,载气(N2)流速为2.5L/min。结果:白花丹叶中β-谷甾醇的含量最高,其次是茎,根中含量最少;3、5、7、8月份采收的药材中含量最高,11、12月最低。结论:以β-谷甾醇为主要成分指标时最佳入药部位为叶,最佳采收期在3、5、7、8月份。Objective: To determine the content of β-sitosterol in the root,stem,and leaves of 1-12 month-old wild Plumbaginis Zeylanicae.Methods: HPLC was used to separate and determine the content of β-Sitosterol in the Plumbaginis Zeylanicae.Chromatographic conditions was that the mobile phase was pure methanol,the column Dumas C18(250 mm × 4.6 mm,5 μm),flow rate 1.0 mL/min;column temperature at 25 ℃.ELSD parameters was the drift tube at 65 ℃ and the flow rate of carrier gas(N2) was 2.5 L/min.Results: The content of the β-sitosterol was highest in leaves,followed by the stems and the roots at least.Optimal harvest time was in March.,May,July and August and the leaves in November and December had the lowest content.Conclusion: The best medical part of the plant was the leaves,considering the content of the β-sitosterol as the main ingredient,and the best harvest time was in March,May,July and August.Plumbaginis zeylanicae leaves can be used as a new drug resources plant for chlorogenic acid.

关 键 词：野生白花丹 Β-谷甾醇 含量测定 RP-HPLC-ELSD 

分 类 号：R284[医药卫生—中药学]