RIP140基因多态性及其与济宁青山羊产羔数关系的研究  

Polymorphism of RIP140 gene and its relationship with litter size of Jining Grey goat

作　　者：唐倩倩[1] 冯涛[1] 储明星[1] 狄冉[1] 方丽[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与种质创新重点实验室,北京100193

出　　处：《安徽农业大学学报》2012年第1期14-18,共5页Journal of Anhui Agricultural University

基　　金：国家高技术研究发展计划(863)(No.2006AA10Z139);国家现代农业产业技术体系建设专项资金(CARS-39);国家重点基础研究发展计划(973)(No.2006CB102105);国家自然科学基金项目(No.30871773);中国农业科学院北京畜牧兽医研究所基本科研业务费专项(2010jc-9);中国农业科学院首批优秀科技创新团队专项共同资助

摘　　要：根据牛RIP140基因mRNA序列,设计5对引物(P1～P5),利用每对引物分别扩增随机选取的济宁青山羊和辽宁绒山羊各5个个体,PCR产物克隆测序,获得山羊RIP140基因全部CDS序列。通过序列比对在此序列822位发现C→T的沉默突变。根据获得的山羊RIP140基因的CDS序列设计引物P6,采用PCR-RFLP技术检测C822T多态位点在高繁殖力品种(济宁青山羊、贵州白山羊)和低繁殖力品种(辽宁绒山羊、波尔山羊、内蒙古绒山羊)中的单核苷酸多态性,并研究该基因对山羊产羔数的影响。结果发现,引物P6扩增片段在济宁青山羊、贵州白山羊、辽宁绒山羊和波尔山羊中均检测到CC、CT和TT 3种基因型,内蒙古绒山羊中只检测到CT和TT两种基因型。济宁青山羊CC、CT和TT基因型频率分别为0.056、0.309和0.635,CC型和CT型母羊平均产羔数分别比TT型多0.81只(P<0.05)和0.65只(P<0.05),CC型比CT型多0.16只(P>0.05)。研究结果初步表明,RIP140基因的C等位基因是提高山羊产羔数的一个潜在有效的DNA标记。According to the Bos taurus RIP140 mRNA sequence,five pairs of primers were designed to am-plify whole CDS sequence of RIP140 gene in five randomly selected individuals from Jining Grey goats and Liaoning Cashmere goats,respectively.Through DNA sequencing and alignment,the whole CDS sequence of the goat RIP140 gene was obtained.Meanwhile,one nucleotide transition（C822T） was identified in goat RIP140 gene.According to the CDS sequence of the goat RIP140 gene,primer P6 was designed to detect C822T mutation of RIP140 gene in high prolificacy breeds（Jining Grey and Guizhou White goats） and low prolificacy breeds（Liaoning Cashmere,Boer and Inner Mongolia Cashmere goats） by PCR-RFLP.The results showed that three genotypes（CC,CT and TT） were detected in Jining Grey,Boer,Liaoning Cashmere and Guizhou White goats and only two genotypes（CT and TT） were detected in Inner Mongolia Cashmere goat.In Jining Grey goat,genotype frequency of CC,CT and TT was 0.056,0.309 and 0.635,respectively.Jining Grey goat does with genotype CC or CT had 0.81（P〈0.05） or 0.65（P〈0.05） kids more than those with genotype TT,respectively.The Jining Grey goat does with genotype CC had 0.16（P〉0.05） kids more than those with genotype CT.These results preliminarily indicated that allele C of RIP140 gene is a potential DNA marker for improving litter size in goats.

关 键 词：山羊 RIP140 高繁殖力 PCR-RFLP 

分 类 号：S827[农业科学—畜牧学]