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机构地区:[1]西南林业大学,云南昆明650224 [2]德宏州林业局中心苗圃,云南德宏678400
出 处:《安徽农业科学》2012年第6期3267-3268,共2页Journal of Anhui Agricultural Sciences
基 金:国家科技支撑计划项目"西南高山区油茶丰产栽培技术集成与示范"(2009BADB1B08);云南省重点新产品开发计划项目"油茶优良品种选育"(2009BB004)
摘 要:[目的]对德宏州油茶落花落果造成油茶低产的原因进行初步研究。[方法]使用碘-碘化钾和23,5,-氯化三苯基四氮唑法测量花粉的活力,用离体培养方法检测花粉的萌发率,用苯胺蓝染色法观察花粉管生长情况,分析不同授粉方法对油茶坐果率的影响。[结果]与结实差的植株相比,结实好的普通油茶花的花粉活力和花粉管数量明显较高,但它们的花粉萌发率无显著差异;异花授粉有利于油茶的坐果。[结论]油茶花粉的生长发育情况对德宏油茶的产量有重要影响。[Objective] The paper was to preliminarily investigate the low yield reason of Camellia oleifera caused by fruit and flower dropping in Dehong prefecture.[Method]Pollen viability of C.oleifera was determined using I-KI and TTC(2,3,5-triphenyl tetrazolium chloride) method,the germination rate of pollen was determined by in vitro culture method,the pollen tube growth was observed by the fluorescent microscope through aniline blue dyeing,and the effects of different pollination methods on fruit setting rate of C.oleifera was analyzed.[Results] The pollen viability and the quantity of pollen tube of C.oleifera with good fruiting were significantly higher than the plants with poor fruiting,but their pollen germination rate had no significant differences;cross pollination was conducive to fruiting setting of C.oleifera.[Conclusion] The growth and development condition of C.oleifera pollen had important effect on the yield of C.oleifera in Dehong prefecture.
分 类 号:S727.32[农业科学—林木遗传育种]
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