草莓镶脉病毒(SVBV)ORFⅠ基因的克隆及序列分析  

Cloning and Sequence Analysis of Gene ORFⅠ of Strawberry vein banding virus(SVBV)

作　　者：贾琳[1] 倪方锐[1] 李瑞[1] 蒋磊[1] 丁菲[1] 江彤[1] 

机构地区：[1]安徽农业大学植物保护学院,合肥230036

出　　处：《吉林农业大学学报》2012年第1期62-65,共4页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(30740033);安徽省教育厅自然科学基金重点项目(KJ2009A105;KJ2008A133)

摘　　要：用CTAB法从感染草莓镶脉病毒(SVBV)的草莓叶片中提取总DNA,设计特异性引物扩增含有SVBVORFⅠ的片段,克隆并测序。序列分析表明,该片段全长1 033个核苷酸,其中包含的ORFⅠ全长987个核苷酸,编码328个氨基酸。结果表明:将其与花椰菜花叶病毒属其他成员的ORFⅠ序列相比较,中国SVBVORFⅠ与美国SVBV ORFⅠ序列相似性最高,达88.3%。进一步构建SVBV及其同属其他成员ORFⅠ的系统关系树,结果显示:中国SVBV ORFⅠ与美国SVBV ORFⅠ单独形成1个亚分支,说明来源于草莓的2个SVBV亲缘关系最近。The total DNA was extracted from strawberry leaves infected with SVBV by CTAB method. Specific primer pair was designed to amplify the fragment including SVBV ORF I , and then the fragment was cloned and sequenced. Sequence analysis results showed that the fragment was compzosed of 1 033 nts, including 987 nts of full-length ORF I gene encoding 328 amino acids. Comparing the sequence of SVBV ORF I with that of the ORF I of other members of Caulimovirus, the result showed that Chinese SVBV ORF I shared the highest sequence similarity （88.3%） with that of the American SVBV. A phylogenetic tree based on alignment of the nucleotide sequences of ORF I of SVBV and other members of Caulimovirus was constructed. The result indicated that the Chinese SVBV ORF I and the American SVBV ORF I clustered into a separate subgroup. It illustrated that the two SVBV derived from strawberry had the closest relationship

关 键 词：草莓镶脉病毒 ORFⅠ 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]