大鼠小肠移植慢性排斥模型的建立  

Establishment of small bowel transplant model of chronic rejection in F344-to-Lewis rats

作　　者：张海云[1] 许荣誉[1] 庄奕煌[1] 李新宇[1] 庄建良[1] 

机构地区：[1]福建医科大学附属泉州第一医院肿瘤外科,泉州362000

出　　处：《海峡药学》2012年第1期34-37,共4页Strait Pharmaceutical Journal

摘　　要：目的建立稳定可靠的大鼠小肠移植慢性排斥模型。方法供受体分别为雄性近交系F344,Lewis大鼠。Ⅰ组为同基因移植对照组,Lewis-to-Lewis,n=8;Ⅱ组为异基因移植组,F344-to-Lewis,n=12。术后2,6,8,12周经造瘘口取材,组织病理学分析。结果异基因组移植肠术后6~12周显示典型的慢性排斥表现。术后越晚期病理特征越明显,但是8周的病理特征与12周比较无显著性差异。结论该模型是稳定可靠的小肠移植慢性排斥模型。OBJECTIVE For the purpose of immunological study on chronic rejection, we establish a rat model of small bowel transplantation. METHODS Heterotopic small bowel transplantations were performed from F344 or Lewis donors to Lewis recipients. The recipients were divided into 5 groups, namely, isograft group （Group I , n = 6） and allograft groups （Group Ⅱ, Group Ⅲ, Group Ⅳ, Group V, n = 24）. All recipients were treated with lowdose Cyclosporine A （5mg/kg/d） from 0-13 days after transplantation. Sequential biopsies were performed at 4, 6, 8 and 12 weeks post-transplantation from Group Ⅱ, Ⅲ, IV and V recipients and all recipients were sacrificed at 12 weeks post-transplantation. Graft arteriosclerosis, fibrosis, inflammatory infiltration and blunting of villi were evaluated. RESULTS The histological features of CR in allografts were fully developed from 6 to 12 weeks after transplantation. Although the features displayed much more obviously with time, they attained no significance between 8 week and 12 week after transplantation.CONCLUSIONS Small bowel transplant model in F344-to- Lewis rat is a useful and reliable model which displays significant features of CR around 8 weeks post-transplant.

关 键 词：大鼠 小肠移植 慢性排斥 

分 类 号：R969[医药卫生—药理学]