两种大叶钩藤ITS序列的RFLP分析  

ITS sequence RFLP analysis of two Uncaria macrophylla Wall from different regions

作　　者：朱爽[1] 陈剑平[1] 刘嘉敏[1] 周林[1] 孙悦[2] 曾常青[2] 

机构地区：[1]广东药学院生命科学与生物制药学院,广州510006 [2]广东药学院中药学院,广州510006

出　　处：《海峡药学》2012年第1期42-44,共3页Strait Pharmaceutical Journal

基　　金：广东省自然科学基金资助项目(8451022401001607)

摘　　要：目的通过对两种不同产地大叶钩藤(Uncaria macrophylla Wall.)的核糖体内转录间隔区(rDNA ITS)序列进行RFLP遗传分析,研究不同地理区域大叶钩藤的rDNA ITS序列是否具有生物地理上的差异。方法通过CTAB法提取样品DNA,利用通用引物对其rDNA ITS进行PCR扩增,从而对连接转化后扩增得到的rDNA ITS序列进行限制性片段长度多态性(Restriction Fragment Length Polymorphism,RFLP)分析。结果获得采集自广东肇庆鼎湖山和广西植物园的两种大叶钩藤两种限制性内切酶(MspⅠ&HaeⅢ)酶切图谱。结论基于对rDNA ITS序列进行的RFLP分析,表明这两个地区的大叶钩藤rDNA ITS序列并无明显差异,不同地理区域的大叶钩藤其遗传本质不具有地理差异。OBJECTIVE In order to demonstrate the phylla Wall, genetic analysis of two Uncaria macrophylla geographical and biologic differences of Uncaria macroWall with different regions was carried out. METHODS Firstly, Total DNA was extracted with CTAB method. Secondly, rDNA ITS regions were amplified with universally conserved primer. Finally, the rDNA ITS amplicon was characterized by RFLP （Restriction Fragment Length Polymorphism, RFLP）analyses. RESULTS The Msp I-and Hae Ⅲ-based RFLP patterns of rDNA ITS from two kinds of Uncaria macrophylla Wall were obtained, which collected from Dinghushan biosphere reserve, Zhaoqing City, Guangdong and Guangxi Botanical Garden. CONCLUSION Based on molecular biology methods of rDNA ITS region RFLP analysis, the two regions of Uncaria macrophylla Wall have no Significant geographical differences.

关 键 词：大叶钩藤 ITS序列 RFLP分析 

分 类 号：R282.71[医药卫生—中药学]