晚期糖基化终产物对内皮细胞Connexin40基因表达的影响  

Advanced glycation end products down-regulate the expression of Connexin40 gene in cultured human umbilical vein endothelium cells

作　　者：章顺荣[1] 高越[1] 洪鸣鸣[1] 封菲[1] 王晓楠[1] 周叶[1] 

机构地区：[1]杭州市第一人民医院老年医学科,310006

出　　处：《浙江医学》2012年第1期22-25,共4页Zhejiang Medical Journal

基　　金：浙江省医药卫生科技计划项目（2008B145）

摘　　要：目的研究晚期糖基化终产物（advanced glycation end products，AGEs）对体外培养的人脐静脉内皮细胞缝隙连接蛋白Connexin40基因表达的影响。方法D-葡萄糖孵育牛血清白蛋白制备糖基化牛血清白蛋白作为晚期糖基化终产物。体外常规培养的人脐静脉内皮细胞分为4组，分别为对照组、100mg／LAGEs干预组、200mg／LAGEs干预组和400mg／LAGEs干预组，干预24h后通过RT—PCR方法检测各组Connexin40基因mRNA的表达有无差异；通过免疫细胞荧光方法检测对照组和200mg／LAGEs干预组之间Connexin40基因蛋白的表达水平有无差异。结果不同浓度（100、200、400mg／L）的AGEs干预24h均能引起人脐静脉内皮细胞Connexin40基因mRNA的表达减少（均P〈0．01）；200mg／L的AGEs干预24h引起人脐静脉内皮细胞Con—nexin40基因的蛋白表达减少（P〈0．01）结论AGEs体外短期干预可引起人脐静脉内皮细胞Connexin40基因的mRNA和蛋白表达水平减少。Objective To investigate the effect of advanced glycation end producs （AGEs） on the expression of gap junction protein connexin40 gene in cultured human umbilical vein endothelium cell （HUVEC） in vitro. Methods Bovine serum albumin （BSA） was incubated with D-glucose to produce AGE-BSA. Human umbilical vein endothelial cells cultured in normal condition were divided into blank control group, 100rag/L, 200mg/L and 400mg/L AGEs intervention groups, 24h after AGEs treatment, the expression of connexin40 mRNA of cultured HUVEC was detected with RT-PCR method; while the expression level of connexin40 protein was detected with immunofluorescence method in the control and 200mg/L AGEs intervention groups. Results AGEs （ 100mg/L, 200mg/L and 400rag/L） down-regulated the expression levels of connexin40 mRNA in cultured HUVEC after 24h intervention （P〈0.01）; connexin40 protein level in cultured HUVEC of 200 mg/L AGEs group was decreased compared with the control group （P〈0.01）. Conclusion AGEs may down-regulate the protein and mRNA expression level of connexin40 gene in cultured human vein endothelium cell in vitro, which indicates that connexin40 may be involved in the pro-atherosclerotic effect of AGEs.

关 键 词：缝隙连接蛋白 Connexin40 晚期糖基化终产物 人脐静脉内皮细胞 基因表达 

分 类 号：R285.5[医药卫生—中药学]