柯萨奇B3病毒感染与HL-1心肌细胞凋亡的相关性  

Association of coxsackievirus B3 infection with apoptosis of HL-1 cells

作　　者：彭俊[1] 钱正明[1] 黄建振[1] 高峰[1] 高世龙[1] 

机构地区：[1]杭州市萧山区第一人民医院心内科,311200

出　　处：《浙江医学》2012年第1期32-33,38,共3页Zhejiang Medical Journal

摘　　要：目的探讨柯萨奇B3病毒感染与HL-1心肌细胞凋亡的相关性。方法通过实时荧光PCR方法检测柯萨奇B3病毒感染4、12、24h及未感染的HL-1心肌细胞Bcl-2及Bax的表达，同时采用AnnexinV—PE凋亡检测试剂盒检测HL-1心肌细胞的凋亡率。结果柯萨奇B3病毒感染后4、12、24h细胞凋亡率均显著高于对照组（P〈0．01），感染后4h细胞凋亡率显著高于感染后12、24h（P〈0．01）。与对照组比较，柯萨奇B3病毒感染后4、24h的Bcl-2／Bax均明显变大（P〈O．05或0．01），而感染后12h的Bcl-2／Bax无明显变化（P〉0．05）；感染后4h的Bcl-2／Bax显著大于感染后24h（P〈0．01）。结论柯萨奇B3病毒可导致HL-1心肌细胞的凋亡，在感染后4h其作用尤为明显。Objective To investigate the association of Coxsackievirus B3 infection with apoptosis of myocardial HL-1 cells. Methods The expression of Bcl-2 and Bax in HL-1 cells was detected by real time PCR, and apoptosis rate was examined by Annexin V-PE kit. Results Compared with non-infected HL-1 cells, the expression of Bcl-2/Bax in infected HL-1 cells increased significantly （P〈0.05）at 4h and 24h after infection; while Bcl-2/Bax expression in cells at 4h after infection was higher than that at 24h after infection （P〈0.05）. Compared with non-infected HL-1 cells apoptosis rate in infected cells increased sig- nificantly（P〈0.05）; while the apoptosis rate in HL-1 at 4h after infection significantly higher than those at 12h and 24h after infec- tion（P〈0.05 ）. Conclusion Coxsackievirus B3 infection contributes to the apoptosis of HL-1 cells, especially 4h after infection.

关 键 词：柯萨奇B3病毒 HL-1 心肌细胞 凋亡 

分 类 号：R373.23[医药卫生—病原生物学]