全反式维甲酸在帕金森病大鼠模型中脑神经干细胞移植治疗中的作用  

Transplantation of all-trans retinoic acid-treated midbrain-derived neural stem cell for Parkinson＇s disease in rats

作　　者：盛汉松[1] 周辉[1] 许尚虞[1] 林坚[1] 尹波[1] 张弩[1] 

机构地区：[1]温州医学院附属第二医院神经外科,325027

出　　处：《浙江医学》2012年第2期99-102,共4页Zhejiang Medical Journal

基　　金：浙江省科技厅科研基金资助项目（2007C23026）;浙江省卫生厅科研基金资助项目（2007B146）;温州市科技局科研基金资助项目（Y20070042）

摘　　要：目的 观察全反式维甲酸（atRA）处理的大鼠中脑神经干细胞（NSCs）移植对帕金森病（PD）模型大鼠的治疗作用.方法 制备PD大鼠模型并将成功模型分为对照组、常规培养中脑NSCs组和atRA处理中脑NSCs组（atRA＋NSCs组）.动态观察模型大鼠NSCs移植前后的行为学变化,定量分析纹状体多巴胺能神经元表达的变化.结果 atRA＋NSCs组与对照组、NSCs组比较,实验动物的行为功能指标均得到有效改善（P＜0.05或0.01）.atRA＋NSCs组与NSCs组大鼠纹状体移植区BrdU标记的酪氨酸羟化酶染色阳性细胞（TH＋细胞）分别为（261.2±31.7）、（204.3±25.1）个,差异有统计学意义（P＜0.05）,而对照组纹状体区没有BrdU标记TH＋细胞.结论 atRA在PD大鼠模型中脑NSCs移植治疗中有明显的促进作用,具有潜在的临床应用价值.Objective To evaluate the transplantation of stem call （NSC） for Parkinson＇s disease （PD） in rats. Methods all-trans retinoic acid （atRA）- treated midbrain-derived neural The PD model was induced by injection of 6-hydroxydopamine （6-OHDA） in right striatum in rats. The PD rats were divided into 3 groups： control group, NSC group （transplantation of midbrain-derived NSCs ） and atRA＋NSCs group （transplantation of atRA- treated NSCs ）. The rotation scores were assessed before and 1, 4, 8, 12 weeks after transplantation. The dopaminergic neurons in striatum were analyzed quantitatively using immunohistochemistry for tyrosine hydroxylase（TH ）. Results The apomorphine-induced rotation in atRA＋NSCs group was decreased sig- nificantly compared with that of NSCs group （P〈0.05） and control group （P〈0.01）. TH-positive neurons emerged in the striatum of atRA＋NSCs and NSCs group was 261.2 ± 31.7 and 204.3 ± 25.1 （P〈0.05） after transplantation, but no TH-positive neurons were found in the control group. Conclusion All-trans retinoic acid can enhance the efficacy of NSC transplantation for treatment of PD in rats.

关 键 词：全反式维甲酸 PD 神经干细胞 移植 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学]