Differences in lead isotopic fingerprints between blood, hair and organs in lead-poisoned rats  

作　　者：ZENG Jing YAN LaiLai OUYANG Li WU Jing WANG JingYu 

机构地区：[1]School of Public Health,Peking University,Beijing 100191,China [2]Center of Medical&Health Analysis,Peking University,Beijing 100191,China

出　　处：《Chinese Science Bulletin》2012年第7期744-749,共6页

基　　金：supported by the National Natural Science Foundation of China (30972434);the National Science and Technology Major Project (2009ZX09502-027-4)

摘　　要：Lead isotopic ratios of biosamples such as blood, hair and teeth are used as "fingerprints" to trace and identify sources of lead pollution in the environment. However, the reliability of this technique has not been confirmed. In previous studies, we investigated the differences in lead isotopic ratios between blood, hair, and organs from the same human body. To explore the relation-ship between isotopic ratios in environmental lead and biosamples, and to investigate the influence of exposure dose, rats were exposed to 0, 30, 60, and 120 mg/kg body weight of lead acetate every day for 4 weeks by intragastric administration. Lead iso- topic ratios of blood, hair, liver, kidney and femur in rats, as well as that of the test substance lead acetate, were measured using inductively coupled plasma mass spectrometry. There were significant differences (P<0.05) in lead isotopic fingerprints between blood, hair, and tissues. Lead fingerprints of blood were influenced by lead exposure dose, while those of other tissues and organs were generally not. Its reliability and availability indicated that blood, but not hair, was thus a suitable biomarker for environmental lead exposure. The results of this study provide the scientific rationale for the use of lead isotopic ratios for the identification of human lead pollution sources.Lead isotopic ratios of biosamples such as blood, hair and teeth are used as ＂fingerprints＂ to trace and identify sources of lead pollution in the environment. However, the reliability of this technique has not been confirmed. In previous studies, we investi- gated the differences in lead isotopic ratios between blood, hair, and organs from the same human body. To explore the relation- ship between isotopic ratios in environmental lead and biosamples, and to investigate the influence of exposure dose, rats were exposed to 0, 30, 60, and 120 mg/kg body weight of lead acetate every day for 4 weeks by intragastric administration. Lead iso- topic ratios of blood, hair, liver, kidney and femur in rats, as well as that of the test substance lead acetate, were measured using inductively coupled plasma mass spectrometry. There were significant differences （P〈0.05） in lead isotopic fingerprints between blood, hair, and tissues. Lead fingerprints of blood were influenced by lead exposure dose, while those of other tissues and organs were generally not. Its reliability and availability indicated that blood, but not hair, was thus a suitable biomarker for environmen- tal lead exposure. The results of this study provide the scientific rationale for the use of lead isotopic ratios for the identification of human lead pollution sources.

关 键 词：铅同位素比值 人体器官 血液 指纹 头发 大鼠 电感耦合等离子体 中毒 

分 类 号：X503.1[环境科学与工程—环境工程] Q811[生物学—生物工程]