Effect of acetazolamide on stable carbon isotope fractionation in Chlamydomonas reinhardtii and Chlorella vulgaris  

作　　者：WU YanYou XU Ying LI HaiTao XING DeKe 

机构地区：[1]State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550002,China [2]Graduate University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Chinese Science Bulletin》2012年第7期786-789,共4页

基　　金：supported by the National Natural Science Foundation of China (40973060 and 31070365)

摘　　要：The effect of extracellular carbonic anhydrase (CAex) on stable carbon isotope fractionation in algae is still unclear. The stable carbon isotope composition and algal growth in the presence and absence of the membrane-impermeable CA inhibitor acetazolamide were compared in Chlamydomonas reinhardtii and Chlorella vulgaris. The CAex of both algal species contributed about 9‰ of the stable carbon isotope fractionation and exhibited a dosage effect. Therefore, evidence in vivo that CAex leads to a larger carbon isotope fractionation of algae is presented.The effect of extracellular carbonic anhydrase （CAex） on stable carbon isotope fractionation in algae is still unclear. The stable carbon isotope composition and algal growth in the presence and absence of the membrane-impermeable CA inhibitor acetazola- mide were compared in Chlamydomonas reinhardtii and Chlorella vulgaris. The CAex of both algal species contributed about 9%0 of the stable carbon isotope fractionation and exhibited a dosage effect. Therefore, evidence in vivo that CAex leads to a larg- er carbon isotope fractionation of algae is presented.

关 键 词：稳定碳同位素组成 同位素分馏效应 小球藻 乙酰唑胺 衣藻 藻类生长 碳酸酐酶 剂量效应 

分 类 号：Q949.217[生物学—植物学] S791[农业科学—林木遗传育种]