不同根瘤菌株对半无叶型豌豆生育特征、产量及品质的影响  

Effects of inoculation with different Rhizobium leguminosarum strains on growth performances,yield,and grain quality of a semi-leafless pea variety

作　　者：郭丽梅[1] 陈恭[1] 隋新华[2] 张世晨[1] 杨亚东[1] 龙金成[1] 胡跃高[1] 曾昭海[1] 

机构地区：[1]中国农业大学农学与生物技术学院/农业部农作制度重点开放实验室,北京100193 [2]中国农业大学生物学院/农业部农业微生物资源与应用重点实验室,北京100193

出　　处：《中国农业大学学报》2012年第1期46-52,共7页Journal of China Agricultural University

基　　金：国家自然科学基金资助项目(30871491;31171509);国家"十二五"科技支撑计划项目(2011BAD16B15);公益性行业(农业)科研专项(201103001)

摘　　要：在盆栽条件下,对加拿大豌豆品种M.P.1824接种8株豌豆根瘤菌菌株,研究其对豌豆生育特征、产量及品质的影响。结果显示:与不接种对照相比,接种根瘤菌可以延长豌豆生育期,促进后期豌豆植株生长,提高产量,增加籽粒粗蛋白质、粗纤维含量,以及降低可溶性糖含量。其中,接种菌株ACCC 16058豌豆初花期、盛花初荚期、盛荚期及成熟期分别推迟3.1、5.2、4.8及12d,盛花初荚期分枝数增加45.5%,成熟期株高增加16.61%,单株荚数、单株粒数、单株籽粒干重及单盆籽粒干重分别提高76.67%、85.54%、65.13%和36.71%,均存在显著差异(P<0.05)。接种商用菌株F98,可显著提高籽粒粗蛋白质含量与降低籽粒可溶性糖含量(P<0.05),增减量分别为+12.37%、-37.77%;接种CCBAU 43228和CCBAU 43232的籽粒粗纤维含量分别增加1.24和1.20倍,且比F98菌株增加39.7%和37.6%,均达极显著水平(P<0.01);其中接种CCBAU 43232处理可溶性糖含量降低35.65%,差异达显著水平(P<0.05)。综合分析表明,M.P.1824盆栽条件下接种菌株ACCC 16058表现最佳。In order to study the effects of inoculation with Rhizobium leguminosarum strains on growth performances,yield,and grain quality of Canadian pea M.P.1824,a pot experiment was conducted involving inoculating with eight different Rhizobium leguminosarum strains.The results showed that,compared with no-inoculated control,rhizobium inoculation could extend pea＇s growth periods,produce a better growth in pea＇s ripening period,increase yield,and increase grain crude protein and fiber content,but reduce the content of soluble sugar.When inoculated with rhizobium ACCC 16058,periods of flowering beginning,flowering and podding beginning,podding,pod ripening delayed by 3.1,5.2,4.8,and 12 d,respectively.The number of branches at flowering and podding beginning period and plant height at pod ripening period increased by 45.5% and 16.61% respectively.In addition,pods per plant,grains per plant,grain dry weight per plant,and grain dry weight per pot were significantly（P0.05） increased by 76.67%,85.54%,65.13%,and 36.71%,respectively.Inoculating with commercial rhizobium F98 significantly（P0.05） raised the crude protein content and reduced the soluble sugar content,with variable quantity of ＋12.37% and-37.77%.Inoculating with CCBAU 43228 and CCBAU 43232 significantly（P0.01） increased the content of crude fiber by 1.24 and 1.20 times compared with CK,and by 39.7% and 37.6% compared with F98.While inoculating with CCBAU 43232 decreased the content of soluble sugar by 35.65%.In conclusion,for pot experiment,the comprehensive performance of M.P.1824 inoculated with rhizobium ACCC 16058 was the best.

关 键 词：豌豆根瘤菌 半无叶型豌豆 生育特征 产量 品质 

分 类 号：Q939.9[生物学—微生物学]