HPLC-ELSD法测定消栓通络胶囊中三七皂苷R_1、人参皂苷Rg_1和人参皂苷Rb_1的含量  

Determination of notoginsenoside R_1,ginsenoside Rg_1 and ginsenoside Rb_1 in Xiaoshuan Tongluo Jiaonang by HPLC-ELSD

作　　者：曾岗[1] 李慧敏 

机构地区：[1]华容县人民医院,湖南岳阳414200 [2]岳阳市药品检验所,湖南岳阳414000

出　　处：《中南药学》2012年第1期16-19,共4页Central South Pharmacy

摘　　要：目的采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法测定消栓通络胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量。方法以乙腈-水为流动相梯度洗脱(0～12min,水80%→65%),Hypersil ODS柱(4.6mm×250mm,5μm)为固定相,流速为1.0mL.min-1,ELSD参数:漂移管温度:45℃,载气流量:1.5L.min-1。结果三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的回收率分别为96.7%、96.4%和97.7%,RSD分别为1.6%、1.4%和2.2%。结论该方法简便、快速、重现性好,可用于消栓通络胶囊的质量控制。Objective To develop an HPLC-ELSD method for the content determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Xiaoshuan Tongluo Jiaonang. Methods Hypersil ODS analytical column(4.6 mm×250 mm,5 μm) was used with the mobile phase consisting of acetonitrile-water in gradient elution(0-12 min,water 80%→65%).The detector drift tube temperature was 45 ℃ and the nitrogen flow rate was 1.5 L·min-1. Results The recoveries for notoginsenoside R1,ginsenoside Rg1,and ginsenoside Rb1 were 96.7%,96.4%,and 97.7% with RSD 1.6%,1.4%,and 2.2%,respectively. Conclusion The method is simple,rapid,repeatable,and can be used for the quality control of Xiaoshuan Tongluo Jiaonang.

关 键 词：消栓通络胶囊 高效液相色谱-蒸发光散射检测器 三七皂苷R1 人参皂苷Rg1 人参皂苷Rb1 

分 类 号：R927.2[医药卫生—药学] R917