醇相体系制备高纯甘油磷脂酰胆碱的研究  

Preparation of High Purity L-alpha Glycerylphosphorylcholine Catalyzed by Sodium Methoxide in Alcohol Phase

作　　者：张康逸[1] 刘元法[1] 周丽[1] 钱祥云 王兴国[1] 

机构地区：[1]江南大学食品学院食品科学与技术国家重点实验室,无锡214122 [2]常熟富士莱化工有限公司,苏州215558

出　　处：《中国粮油学报》2012年第2期36-41,共6页Journal of the Chinese Cereals and Oils Association

基　　金：863计划(2010AA101506)

摘　　要：探讨了在醇相体系中甲醇钠催化制备甘油磷脂酰胆碱(L-alpha glycerylphosphorylcholine)的可行性,建立了磷脂酰胆碱(phosphatidylcholine)和L-α-GPC的HPLC-ELSD检测方法,研究了温度、料液比、甲醇钠添加量、反应时间对PC转化率和GPC得率的影响。利用Design Expert软件对工艺参数进行优化,得最佳条件:温度35℃、料液比3/20(g/mL)、甲醇钠添加量2 mL、反应时间1.5 h,GPC得率为88.2%。极差分析结果,反应条件对GPC得率影响的顺序为:甲醇钠添加量>温度>反应时间>料液比。大豆粉末磷脂的醇解反应液经过硅胶柱色谱纯化,最终可以得到纯度为99.8%的L-α-GPC,回收率78.4%,这为工业级规模制备高纯L-α-GPC提供了一种好的思路和方法。L-alpha Glycerylphosphorylcholine（L-α-GPC） was prepared by alcoholysis of soybean power lecithin with sodium methoxide in methanol phase.A rapid and accurately quantitative method was established for the determination of phosphatidylcholine（PC） and L-α-GPC by HPLC-ELSD.The effect of reaction parameters on the conversion of PC and the yield of GPC were discussed.With Orthogonal Experiment methodology by Design Expert 7.5,the optimum conditions were as follows：reaction temperature 35 ℃,solid-liquid ratio 3/20（g/mL）,dosage of sodium methoxide 2 mL/100 mL,reaction time 1.5 h.The results of HPLC-ELSD showed that the yield of GPC reached 88.2%.It is shown from range analysis,dosage of sidium methoxide is the most significantly factor for the yield of GPC,followed by temperature,reaction time,solid-liquid ratio.The alcoholysis reaction solution of soybean powder lecithin was separated by silica gel column chromatography,and obtaining the purity of L-α-GPC with 99.8%.These results provide a good method for preparing high purity L-α-GPC on industrial-scale.

关 键 词：磷脂酰胆碱 甘油磷脂酰胆碱 甲醇钠 醇解反应 硅胶柱色谱 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]