草鱼胸腺素β_(11)的cDNA克隆及序列分析  

Molecular cloning and characterization of thymosin β_(11) cDNA in grass carp

作　　者：辜文博[1] 袁恬[1] 冯军[1] 顾继锐[1,2] 吴江[2] 刘汉元[2] 徐恒[1,2] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064 [2]通威股份有限公司,成都610081

出　　处：《四川大学学报（自然科学版）》2012年第1期193-198,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：通威股份公司科技发展项目-草鱼基因组计划;四川省科技攻关项目(05NG002-002-4)

摘　　要：以前期构建的草鱼肠道cDNA文库中β胸腺素EST序列为基础,应用cDNA末端快速扩增(RACE)技术扩增草鱼胸腺素β_(11)(thymosinβ_(11),Tβ_(11))的cDNA序列全长(NCBI登录号:HM120850),该序列长320 bp,5'非翻译区47 bp,3'非翻译区141 bp,包含一个132 bp的开放读码框,编码43个氨基酸残基,蛋白质等电点为5.17,分子大小为4892 Da.与其他脊椎动物的同源性对比结果显示,草鱼与斑马鱼胸腺素β_(11)的同源性最高,达到93.0%.结构预测显示草鱼胸腺素β_(11)拥有典型的β胸腺素家族典型的二级结构.The thymosin β11 EST sequence was picked out from the cDNA library of Ctenopharyngodon idellus to design the RACE primers. The full length cDNA sequence of thymosinβ11 （GenBank accession number： HM120850） from C. idellus intestine was cloned by rapid amplification of cDNA ends （RACE） method. The full longth of this gene＇s cDNA is 320 bp. It has a 47 bp 5＇-untranslated region （UTR） and a 141 bp 3＇-UTR. The open reading frame （ORF） of this gene＇s cDNA is 132 bp which putatively encodes 43 amino acids with the isoelectric point of 5.17 and molecular weight of 4892 Da. Multiple alignments showed that the deduced amino acid sequence of ffthymosins from grass crap shares a high degree of identity with β-thymosins sequences from other species, and the highest homology specie is Danio rerio, （93.0%）. Secondary structure prediction analysis indicated that secondary structure of C. idellu^s thymosin ,β11 is highly similar with otherβ-thymosins.

关 键 词：克隆 序列分析 β胸腺素 草鱼(Ctenopharyngodon idellus) 

分 类 号：Q78[生物学—分子生物学]