辛伐他汀对高胆固醇血症小鼠肝脏基因表达的影响(英文)  

Effects of simvastatin on the gene expression in liver of hypercholesterolemia mice

作　　者：康耀霞[1,2] 王文瑞 谭雪梅[1] 段向阳[2] 高巍[2] 侯志萍[2] 张义正[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]包头市疾病预防控制中心微检科,包头014030 [3]包头市第三医院骨科,包头市014040

出　　处：《四川大学学报（自然科学版）》2012年第1期205-210,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家科技支撑项目(2007BAD78B03)

摘　　要：采用抑制消减杂交方法结合targeted display法分离鉴定辛伐他汀处理高胆固醇血症小鼠后差异表达的基因,并采用定量RT-PCR方法对utrophin、TNF-α的mRNA转录水平进行了研究.结果表明辛伐他汀处理后7个基因上调表达,19个基因下调表达;这些基因涉及转录、脂质代谢、免疫反应等过程.定量RT-PCR分析显示utrophin与TNF-α的mRNA转录水平降低.Suppression subtractive hybridization （SSH） and targeted display （TD） combined to further identify and validate the differentially expressed genes. Seven genes after simvastatin treatment were upregulated and nineteen were down-regulated. The corresponding genes were found to be involved in transcription, lipid metabolism process, and immune reaction. The transcription of utrophin gene, coding for a cytoskeletal protein and sharing structural and functional homology with dystrophin, was found to be downregulated after simvastatin treatment. Utrophin mRNA levels in the liver were further analyzed using real-time quantitative RT-PCR. Tumor necrosis factor α （TNF-α） was simultaneously examined to get the information of inflammatory cytokines and utrophin. The results show that simvastatin decreases mRNA levels of utrophin and TNF-αin the livers of hypercholesteremic mice.

关 键 词：辛伐他汀 基因差异表达 UTROPHIN TNF-Α 

分 类 号：Q78[生物学—分子生物学] R322.4[医药卫生—人体解剖和组织胚胎学]