功能化纳米支架承载干细胞移植治疗大鼠心肌梗死的研究  

Transplantation of stem cells using functional nanofiber scaffold for treatment of myocardial infarction in rats

作　　者：崔晓军[1] 谢华[1] 谭玉珍[2] 国海东[2] 王存[2] 张剑凯[1] 郭金华[1] 

机构地区：[1]广东医学院基础医学院人体解剖学教研室,广东东莞524808 [2]复旦大学上海医学院人体解剖学与组织胚胎学系,上海200032

出　　处：《心脏杂志》2012年第1期17-22,共6页Chinese Heart Journal

基　　金：湛江市科技攻关计划项目资助(2010C3102004)

摘　　要：目的:探讨功能化纳米纤维支架(NFS)承载骨髓源性心肌干细胞(MCSCs)移植对大鼠心肌梗死(MI)后心功能恢复的影响,寻找一种更适合承载MCSCs治疗MI的可注射性生物材料。方法:通过单细胞克隆培养技术,从骨髓间充质干细胞中筛选具有心肌特异分化潜能的MCSCs,并固相合成自聚多肽。将24只雌性大鼠MI模型随机分为3组,即对照组、MCSCs移植组和支架承载MCSCs移植组,每组8只。细胞移植后4周,用M型超声心动图检测心功能恢复的情况。将心脏组织切片并进行Masson染色,利用图像分析检测胶原纤维的比率。采用原位荧光杂交法标记心肌组织中的Y染色体细胞,并检测心肌肌钙蛋白T(cTnT)的表达。结果:与MCSCs移植组相比,支架承载细胞移植组大鼠的心功能指数明显提高,胶原纤维比率明显减小,Y染色体阳性细胞数明显增多(P<0.05)。结论:支架承载MCSCs移植治疗MI,有利于移植细胞在受损心肌内的存活和向功能性心肌细胞分化,并可促进心功能恢复,其疗效优于单纯干细胞移植。AIM：To explore the effect of the functional nanofiber scaffold（NFS） and marrow-derived cardiac stem cell（MCSC） transplantation on cardiac function after myocardial infarction（MI） so as to provide theoretical and experimental basis for the new nano-material used in clinical stem cell transplantation.METHODS：MCSCs were selected from marrow mesenchymal stem cells with single-cell cloning culture.Self-assembling peptides were designed and synthesized.Thirty minutes after the establishment of acute MI model of female rats,24 rats were divided into three groups and injected at the edge of the infarcted area with PBS,MCSCs,and NFS＋MCSCs,respectively.Four weeks after MI,cardiac function changes were detected by M-mode echocardiography,and the changes of organizational structure were detected by Masson staining.Donor cells in myocardial tissue were marked with Y chromosome through in situ hybridization.The expression of（cardiac troponin T） cTnT of Y chromosome-positive cells was detected by immunohistochemistry.RESULTS：Functional recovery of rat hearts,myocardial scar size reduction and Y chromosome-positive cells of the NFS＋MCSC transplantation group were superior to those in MCSC transplantation group,with significant differences between groups（P0.05）.Y chromosome-positive cells expressed cTnT.CONCLUSION：Injectable self-assembling peptide is conducive to the survival and functional myocardial differentiation of MCSCs in the ischemic myocardium,also inducing angiogenesis and a significant reduction in scar area as well as improving cardiac function.

关 键 词：功能化纳米纤维支架 骨髓源性心肌干细胞 心肌梗死 移植 大鼠 

分 类 号：Q813.1[生物学—生物工程]