38株多重耐药鲍曼不动杆菌超广谱酶基因的研究  

Study on Extended-spectrum β-lactamase Genes in 38 Strains of Multidrug Resistant Acinetobacter Baumannii

作　　者：郭雷静[1] 李鑫[1] 张淑芹[2] 陆思静[2] 

机构地区：[1]辽宁医学院,辽宁锦州121001 [2]辽宁医学院附属第一医院,辽宁锦州121001

出　　处：《中国药房》2012年第10期890-893,共4页China Pharmacy

基　　金：辽宁省教育厅基金(2008402);辽宁省科技厅基金(2009225010-41)

摘　　要：目的:了解辽宁医学院附属第一医院38株多重耐药鲍曼不动杆菌超广谱酶基因存在状况。方法:用微量稀释法测定临床分离的38株多重耐药鲍曼不动杆菌对14种抗生素的耐药性,采用多重聚合酶链反应(PCR)、基因测序技术对鲍曼不动杆菌进行超广谱酶基因测定。结果:38株多重耐药鲍曼不动杆菌对14种抗生素高度耐药。检出TEM基因阳性33株(86.84%)、PER-1基因阳性5株(13.16%)、SHV基因阳性1株(2.63%),未检出CTX、GES和VEB基因;其中5株TEM、PER-1基因均阳性,1株TEM、SHV基因均阳性。结论:我院分离的多重耐药鲍曼不动杆菌携带多种超广谱酶基因;应重视合理使用抗生素,减少多重耐药的产生。OBJECTIVE： To investigate the existence of extended spectrum β-lactamase genes in 38 strains of multidrug resistant Acinetobacter baumannii in the First Affiliated Hostpital of Liaoning Medical University. METHODS： Trace dilution method was used to detect the drug resistance of 38 strains of multidrug resistant A. baumannii against 14 kinds of antimicrobial agents. The extended spectrum β-lactamase genes were detected by using PCR assay and gene sequencing. RESULTS： It was found that 38 strains of multidrug resistant A. baumannii isolated were resistant to 14 different antimicrobial drugs. To the extended spectrum β-lactamase genes tested, the positive strains with TEM, PER-1 and SHV were 33 （86.84%）, 5（13.16% ）, 1 （2.63%）, respectively. Among these strains, 5 strains were positive with both TEM and PER-1, 1 strain positive with both TEM and SHV. CTX, GES and VEB genes were not found in all isolates. CONCLUSION： The extended spectrum β-lactamase genes are varied in A. baumannii from our hospital. Antimicrobial agents should be used rationally to decrease multi-drug resistance.

关 键 词：鲍曼不动杆菌 多重耐药 超广谱酶 基因 

分 类 号：R915[医药卫生—微生物与生化药学] R378[医药卫生—药学]