多囊卵巢综合征患者血清内脂素及TNF-α的水平变化及其意义  

Changes and significances of serum visfatin and TNF-α in patients with polycystic ovary syndrome

作　　者：王月恒[1] 邢毅 王玖玲[1] 代军 李淑玲[1] 

机构地区：[1]山东大学齐鲁医院中西医结合科,济南250012 [2]附属省立医院神经外科,济南250021 [3]齐鲁医院查体中心,济南250012

出　　处：《山东大学学报（医学版）》2012年第2期94-97,101,共5页Journal of Shandong University:Health Sciences

摘　　要：目的探讨血清内脂素(visfatin)、肿瘤坏死因子-α(TNF-α)的水平变化对多囊卵巢综合征(PCOS)发病的意义。方法收集PCOS患者50例(实验组)及对照组患者48例,并按体质量指数(BMI)将其分别分成肥胖(BMI≥25 kg/m2)和非肥胖(BMI<25 kg/m2)两个亚组,其中实验组肥胖者23例,非肥胖者27例,对照组肥胖者20例,非肥胖者28例。取空腹血清,采用酶联免疫吸附法(ELISA)测定血清visfatin水平、放射免疫法测定TNF-α水平,葡萄糖氧化酶法测定空腹血糖(FPG)、化学发光法测定空腹胰岛素(FIN)水平,同时测量身高、体质量,并计算BMI和胰岛素抵抗指数(HOMA-IR)。结果实验组visfatin、TNF-α水平均高于对照组(P<0.05)。实验组肥胖者及非肥胖者visfatin水平均高于对照组非肥胖者,实验组肥胖者visfatin水平高于对照组肥胖者;实验组肥胖者及非肥胖者TNF-α水平分别高于其相应对照组,且非肥胖实验组高于肥胖对照组(P<0.05)。实验组visfatin、TNF-α水平与BMI、HOMA-IR均呈正相关(P<0.05)。结论 PCOS患者血清visfatin、TNF-α水平升高均与胰岛素抵抗密切相关,visfatin、TNF-α可能参与了PCOS患者肥胖和胰岛素抵抗的发生发展。Objective To investigate changes of serum visfatin and tumor necrosis factor-α （TNF-ct）in patients with polycystic ovary syndrome（PCOS） and their significances in pathogenesis of PCOS. Methods 50 cases of PCOS patients were collected as the experimental group and 48 healthy subjects as the control group. According to the body mass index（ BMI）, which was calculated by measuring height and body weight, they were subdivided into obese （BMI 〉t25 kg/m2） and nonbese （BMI 〈25 kg/m2） groups, there were 23 cases in the obese experimental group,27 cases in the non-obese experimental group, 20 cases in the obese control group, and 27 cases in the non-obese control group. Fasting serum visfatin was detected by enzyme linked immunosorbent assay（ELISA）, TNF-et by radioimmunoassay （RIA）, fast- ing plasma glucose（FPG） by the glucose oxidation enzyme method, and fasting insulin（FIN） by the electrochemiluminescence immunoassay method. The homeostasis model assessment-insulin resistance（HOMA-IR） was analyzed. Results Visfatin and TNF-α levels in the experimental group were higher than those in the control group（P 〈0.05）. The level of visfatin was higher in both obese and non-obese experimental groups than in the non-obese control group, and it washigher in the obese experimental group than in the obese control group. The level of TNF-α was higher in obese and non-obese patients than in the corresponding control groups, and it was higher in the non-obese experimental group than in the obese control group （ P 〈 0. 05 ）. Visfatin and TNF-a levels were both positively correlated with BMI and HOMA-IR （P 〈 0.05 ）. Conclusion Increases of visfatin and TNF-α levels in PCOS patients are closely related with HOMA-IR. Visfatin and TNF-α play important roles in occurrence and development of obesity and IR in PCOS patients.

关 键 词：多囊卵巢综合征 内脂素 肿瘤坏死因子-Α 胰岛素抵抗 

分 类 号：R711.75[医药卫生—妇产科学]