TC/IC-RT-PCR检测黄瓜绿斑驳花叶病毒  

Detection of cucumber green mottle mosaic virus by TC/IC-RT-PCR

作　　者：沈建国[1] 林双庆[2] 蔡伟[1] 李海明[3] 王念武[1] 翁瑞泉[1] 黄可辉[1] 

机构地区：[1]福建出入境检验检疫局,福建省检验检疫技术研究重点实验室,福建福州350001 [2]福建农林大学生命科学学院,福建福州350002 [3]福建省农业科学院,福建福州350001

出　　处：《福建农林大学学报（自然科学版）》2012年第1期13-17,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省自然科学基金项目(2009J01046);福建省科技计划重点项目(2009N0001);福建出入境检验检疫局科技项目(FK2011-07、FK2008-05、FK2006-19)

摘　　要：根据GenBank上登录的黄瓜绿斑驳花叶病毒(CGMMV)外壳蛋白基因保守序列,设计特异性引物,利用试管捕捉RT-PCR(TC-RT-PCR)和免疫捕捉RT-PCR(IC-RT-PCR)技术对黄瓜绿斑驳花叶病毒进行检测.结果表明,TC-RT-PCR和IC-RT-PCR均具有良好的特异性,检测灵敏度分别比DAS-ELISA高10和100倍;通过对一批进境黄瓜样品的检测,验证了2种检测技术的实际应用效果.因此,本研究建立的TC-RT-PCR和IC-RT-PCR检测技术,可有效应用于CGMMV的快速检测.Specific primers were designed based on the conserved region of coat protein（CP） gene of Cucumber green mottle mosaic virus（CGMMV）.Tube capture RT-PCR（TC-RT-PCR） and immunocapture RT-PCR（IC-RT-PCR） were employed to detect CGMMV.The results showed that two assay technologies had high specificity.Sensitivity provided by TC-RT-PCR and IC-RT-PCR was 10 and 100 times higher than that of DAS-ELISA,respectively.The application effects of two technologies were confirmed by means of detecting CGMMV in imported cucumber samples.TC-RT-PCR and IC-RT-PCR developed in this study can be effectively used for the rapid detection of CGMMV.

关 键 词：黄瓜绿斑驳花叶病毒 试管捕捉RT-PCR 免疫捕捉RT-PCR 

分 类 号：S41-31[农业科学—植物保护]