模拟全血基体中铅及其同位素的富集与分离研究  

Investigation of the Enrichment and Separation of Lead and Lead Isotopes in Simulated Blood Matrix

作　　者：吴晶[1,2] 欧阳荔[1,3] 唐晓萍[4] 刘铁兵[1] 刘多见[1] 王京宇[1,3] 

机构地区：[1]北京大学公共卫生学院,北京100191 [2]河北工业大学化工学院,天津300130 [3]北京大学医药卫生分析中心,北京100191 [4]北京出入境检验检疫局,北京100026

出　　处：《光谱学与光谱分析》2012年第3期765-769,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30972434)资助

摘　　要：利用富集分离方法,克服高浓度基体对铅浓度及铅同位素测定的影响。应用专利方法对模拟全血基体中的铅进行富集分离,用ICP-MS测定富集分离前后的铅浓度、基体浓度及铅同位素比值。富集分离后的铅回收率均在99%以上,对Na和K等主要离子的去除率约为80%;各个铅同位素的浓度值随着基体浓度的增大而减小(p<0.001),但铅各同位素的比值则与基体浓度无关;当基体浓度>62.5μg.mL-1时,各个铅同位素强度会随着测定次数的增加而迅速衰减。富集分离专利方法对复杂基体中痕量铅的回收率高,对杂质离子的去除率较好;明显消除了高浓度基体对ICP-MS进样系统的堵塞现象,操作简便、成本低、引入污染少,可以提高各个铅同位素浓度测定的准确度和精密度,且对铅同位素比值测定准确度无明显影响。The enrichment and separation method was used in the present paper to overcome the influence of matrix effect on the determination of lead concentration and isotope ratios.The patented method was used to enrich and separate the lead in simulated blood matrix;the lead concentrations and isotope ratios were determined by ICP-MS before and after enrichment and separation.The recovery rate of Pb is more than 99% through this method while the removal rate of major impurity ions such as Na,K is about 80%.The concentrations of each lead isotope decrease with the matrix concentration increasing（p0.001）,and lead isotope ratios show no significant differences in the matrix of different concentrations.When the matrix concentration is more than 62.5 μg·mL-1,the strength will decrease with the determination times increasing.It was concluded that the characteristics of this method are of high recovery rate（99%）,fast,simple and convenient,and it can raise the accuracy and precision of the concentration determination of lead isotopes and has no significant influence on the accuracy of lead isotope ratios determination.

关 键 词：富集分离 基体效应 铅 同位素比值 

分 类 号：R313[医药卫生—基础医学]