同种带瓣主动脉和肺动脉移植免疫排斥反应的研究  

Study of immunological rejection in implantation of aortic and pulmonary valved homograft

作　　者：李东玉[1] 张仁福[1] 朱洪玉[1] 宫汉东[1] 王军[1] 李新民[1] 

机构地区：[1]沈阳军区总医院心外科,110015

出　　处：《中华胸心血管外科杂志》2000年第1期25-27,共3页Chinese Journal of Thoracic and Cardiovascular Surgery

摘　　要：目的　探讨同种带瓣管道移植对宿主免疫状态的影响以及排斥反应对移植物的作用。方法　应用同种异体带瓣主动脉或肺动脉重建右室流出道 ,并随机分为A组 (移植组 )、B组 (免疫抑制组 )和C组 (对照组 )。分别在术后 1、2、3、4周收集外周血标本 ,应用流式细胞仪测定B细胞及T淋巴细胞亚群的变化 ,应用酶联法测定血清中IL 2含量。结果　移植 1周后外周血中T淋巴细胞数明显增高 ,CD+ 4阳性率、CD+ 4/CD+ 8比值和IL 2含量明显增高 ,CD+ 8阳性率相对降低 ,B细胞数量没有明显变化。环孢素A治疗对T淋巴细胞激活、IL 2含量增高有明显的抑制作用。结论　低温保存的同种主动脉和肺动脉移植后有明确的免疫排斥反应发生 ,以细胞免疫为主。Objective To study host immune response and effect of immunological rejection on the aortic of pulmonary homografts. Methods Aortic or pulmonary valved homograft was used to reconstruct the right ventricular outflow tract in the treatment of complex congenital heart disease. The blood samples were collected at 1,2,3 and 4 weeks after implantation. The biological activities of IL-2 were measured by means of ELISA. Results The percentage of T lymphocytes and CD + 4 T lymphocytes the radio of CD + 4 to CD + 8, and the biological activities of IL-2 were significantly increased after homograft implantation, and maintained at high activity levels during the 4 weeks after implantation. There was no obvious change in the percentage of B lymphocytes. Treatment with CsA had remarkable suppressive effect on increase of the percentage of CD + 4, and the increase of biological activities of IL-2 after homograft implantation. Conclusion There was significant rejection after implantation of aortic and pulmonary homografts. Cellular immune plays a major role in this reaction. The treatment of CsA has marked suppressive effect on immunological rejection.

关 键 词：同种带瓣主动脉 肺动脉移植 免疫排斥反应 

分 类 号：R654.3[医药卫生—外科学]