输血后丙型肝炎病毒感染的血清病毒定量研究  

Quantitative study of hepatitis C virus RNA in sera of patients with posttransfusion hepatitis C virus infection.

作　　者：王志明[1] 谭复明[1] 陈伟华[1] 李明山[1] 郭素萍[1] 

机构地区：[1]解放军第260医院传染科,河北省石家庄050041

出　　处：《中华传染病杂志》2000年第1期37-39,共3页Chinese Journal of Infectious Diseases

基　　金：全军95 医药攻关课题基金!资助(96L049)

摘　　要：目的　研究血清丙型肝炎病毒(HCV) 含量与HCV 致病的关系及HCV 含量与抗HCV和丙氨酸转氨酶(ALT) 的相关性。方法　以逆转录聚合酶链反应(RTPCR) 法对HCV 感染的受血及相关供血系列血清进行HCVRNA定量分析,同时检测ALT 与抗HCV。结果　致输血后HCV 感染的供血中,HCVRNA 平均含量为108 .6 拷贝/L;抗HCV 及ALT 的异常检出率随HCVRNA 滴度升高而增加。结论　低含量HCV 供血即可导致输血后感染,病毒含量与抗HCV( +) 及ALT 水平有显著正相关,采用ALT 加抗HCV 联合筛检供血可明显降低输血后HCVObjective To study the relationship between the hepatitis C virus (HCV) RNA level the level of alanine aminotransferase (ALT) as well as anti HCV in the sera of HCV infected blood recepients and their related blood donors. Methods The serum HCV RNA was detected by neverse transcription polymetase chain reaction (RT PCR) quantitatively and the levels of ALT and anti HCV were simultaneously detected. Results In the sera of donors which caused posttransfusion HCV infection, the mean level of HCV RNA were 10 8.6 copies/L. The positive rate of anti HCV in serum was increased in the patients with higher HCV RNA level. There was a strong correlaton between the HCV RNA level and ALT value. Conclusion The results suggested that posttransfusion HCV infection could be caused by transfusing blood with low HCV RNA level and exclusion of donated blood for elevated ALT or positive anti HCV could reduce the incidence of posttransfusion hepatitis C infection.

关 键 词：丙型肝炎病毒 HCV 输血后感染 血清病毒 

分 类 号：R512.63[医药卫生—内科学]