温度对DEN-2型病毒在白纹伊蚊体内外潜伏期的影响  

Effect of temperatures on extrinsic incubation period of dengue virus type 2 in Aedes albopictus

作　　者：肖方震[1] 张仪[2] 谢汉国[1] 施文琦[2] 何似[1] 邓艳琴[1] 周晓农[2] 严延生[1] 

机构地区：[1]福建省疾病预防控制中心,福州350001 [2]中国疾病预防控制中心寄生虫病防治研究所,上海200025

出　　处：《中国人兽共患病学报》2012年第2期108-110,共3页Chinese Journal of Zoonoses

基　　金：国家"十一五"科技支撑计划项目(No.2007BAC03A02);国家科技重大专项项目(2008ZX10004-011);福建省社会发展重点项目(2010Y0010)联合资助

摘　　要：目的研究温度对登革2型病毒在蚊体内的外潜伏期的影响。方法登革2型病毒人工经口感染白纹伊蚊,冻麻挑出吸饱血的雌蚊放入新的蚊笼,分别置于18℃、21℃、26℃、31℃和36℃中饲养,第3d、4d、5d、7d、10d、14d、20d、25d分别收集并解剖白纹伊蚊,以间接免疫荧光法分别检测蚊虫头部、唾液腺和胸腹部的登革2型病毒抗原。结果置于18℃、21℃、26℃、31℃和36℃饲养的白纹伊蚊分别在第25d、7d、5d、4d、3d时胸腹部中开始检出阳性。21℃、26℃、31℃和36℃饲养的蚊虫唾液腺阳性检出的时间分别为10d、7d、4d、4d,18℃在感染后的25d内,唾液腺均未检出阳性。结论随着温度的升高,登革2型病毒在白纹伊蚊体内外潜伏期逐渐缩短。To investigate the effect of incubation temperature on extrinsic incubation period, Aedes albopictus were orally infected with dengue virus type 2 suspension, then fully bloodengorged mosquitoes were separated and transferred to new cartons and retained at 18 ℃, 21 ℃, 26 ℃, 31℃ and 36 ℃ with 10%sucrose. Mosquitoes were sacrificed on 3 d, 4 d, 5 d, 7 d, 10 d, 14, 20 d and 25 d postinfection. Immunofluorescence assays were used to detect antigen of dengue virus type 2 in the head, salivary gland and thoraxabdomen. Virus in thoraxabdomen was detected on 25 d,7 d, 5 d,4 d and 3 d postinfection. The extrinsic incubation period was 10 d,7 d,4 d and d4 separately, when mosquitoes were maintained at 21℃,26℃ ,31℃ and 36℃. But virus in salivary was not detected during a 25day incubation period when mosquitoes were retained at 18℃. It was suggested that the extrinsic incubation period of A. albopictus shortened gradually with the increase of extrinsic incubation temperature.

关 键 词：白纹伊蚊 外潜伏期 温度 登革病毒 

分 类 号：R384[医药卫生—医学寄生虫学]