猪狂犬病的确证  

Laboratory-confirmed swine rabies

作　　者：刘祥茵[1] 邓衔柏[1] 施赫赫[1] 范小龙[1] 张莹[1] 郑声陶[1] 郭霄峰[1] 

机构地区：[1]华南农业大学兽医学院,广州510642

出　　处：《中国人兽共患病学报》2012年第2期124-126,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(31172322)资助

摘　　要：目的了解广东省一猪场发生狂犬病猪只的感染状况。方法采集疑似狂犬病发病猪的大脑、小脑以及海马回,制成切片,用姬姆沙染色,在光学显微镜下检测狂犬病病毒包涵体。用抗狂犬病病毒N蛋白荧光抗体染色,在荧光显微镜下检测特异荧光。用蛋白酶K处理病料,抽提核酸,以狂犬病病毒N基因特异引物作RT-PCR检测狂犬病病毒特异性核酸。将病料接种小白鼠接毒,观察小鼠的发病。结果病理学切片检测病猪脑海马神经细胞中有包涵体;脑组织印片经特异性抗狂犬病病毒核蛋白(NP)单克隆抗体免疫荧光染色后,荧光显微镜下可见大量的针尖状绿色荧光颗粒;狂犬病病毒NP特异性目的基因RT-PCR得到与预期结果大小一致的扩增产物;小白鼠接毒试验,3日龄以内乳鼠接毒后出现特异性的神经症状。结论该猪场病死猪检测狂犬病病毒感染阳性,确定猪群中发生狂犬病。To confirm the clinically suspected case of pinges infected with rabies virus in a small hoggery in Guangdong Province, the brain tissue samples including cerebrum, cerebellum and hippocampal gyrus were subiected to Giemsa stain,fluorescent antibody test （FAT） and RTPCR. The samples were inoculated to suckling and adult mice. The results indicated that the inclusion bodies and a lot of green focus could be found in hippocampal gyrus, and a 424bp DNA fragment of rabies virus N gene could be detected from the brain tissues. Between 13 and 15 days postinfection, clinical syptoms of a rabieslike illness developed in all inoculated mice. It was suggested that the pigs in the small hoggery were infected with rabies virus.

关 键 词：狂犬病 猪 包涵体 海马角 

分 类 号：R373.9[医药卫生—病原生物学]