X盒结合蛋白1在高迁移率族蛋白B1诱导树突状细胞成熟分化中的作用  

X-box-binding protein-1 contributes to maturation of splenic dendritic cells induced by high mobility group box-1 protein

作　　者：祝筱梅 姚凤华 姚咏明 董宁 张庆红 于燕 盛志勇 

机构地区：[1]Burns Institute,First Hospited Affiliated to the Chinese PLA General Hospital,Beijing 100048,China

出　　处：《中国急救复苏与灾害医学杂志》2012年第2期152-155,共4页China Journal of Emergency Resuscitation and Disaster Medicine

基　　金：国家自然科学摹金项目（81071545,30800437,81130035,81121004）;闰家重点基础研究发展计划项目（2012CB518102）;创伤、烧伤与复合伤国家重点实验室开放课题（SKLKF201103,SKLKF201002）

摘　　要：目的X盒结合蛋白I（XBP-1）是内质网应激（ERS）的关键调节分子，本研究探讨XBP-1活化在高迁移率族蛋白B1（HMGBl）诱导树突状细胞（Dc）成熟分化中的作用。方法分离正常BALB／c小鼠脾脏DC进行体外培养，给予HMGBI刺激后检测细胞XBP-1活化情况；应用RNAi技术诱导XBP-1基因沉默后检测DC功能状态。结果HMGBl刺激后，XBP-1表达特别是活化水平明显升高（P〈0．05）；XBP-1基因沉默DC给予HMGBl刺激不能有效诱导其成熟分化，表面标志物CD80、CD86及主要组织相容性复合体11分子表达上调受抑，分泌白细胞介素-12、肿瘤坏死因子-α功能增强不明显。结论XBP-1活化对HMGBl诱导DC成熟分化至关重要。Objective To explore the potential role of X-box-binding protein-1 （XBP-1） activating in maturation of splenic dendritic cells （DCs） induced by high mobility group box-1 protein （HMGB1） in vitro as the transcription factor XBP-1 plays an essential role in modulating endoplasmic reticulum stress （ERS） response. Methods DCs were isolated from the spleens of male BALB/c mice, and the ceUs were stimulated with tIMGB1 at different time points. After being stimulated, expression and activation of XBP-1 were analyzed by SYBR green real-time RT-PCR and Western blot. Expressions of costimulatory molecules including CDSO, CD86, major histocompatibility complex （MHC）Ⅱ on surface of DC were determined by flow cytometry. Cytokines in cell culture medium were determined by ELISA. A short hairpin RNA designed against XBP-1 （XBPi） was constructed to induce gene silence of XBP-1. Results HMGB1 induced significant up-regulation of XBP-1 expression and activation in mice splenic DCs （P〈0.05）. Gene silence of XBP-1 in splenic DCs resulted in hindered mature after HMGB1 treatment, when compared with untransfected or nontargeting-transfected DCs （P〈 0.05）. Meanwhile, stimulation with HMGB1 aroused no marked elevation of interleukin-12 or tumor necrosis factor-α production in XBPi-transfeeted DCs as that in normal controls （P〈0.05）. Conclusion These data demonstrated that ERS regulator XBP-1 might contribute to maturation of splenic dendritic cells induced by HMGB1.

关 键 词：高迁移率族蛋白B1 树突状细胞 内质网应激 X盒结合蛋白1 

分 类 号：R64[医药卫生—外科学]