骨收获室内骨细胞的方向性生长  

Orientation growth of osteocyte in the bone harvest chamber

作　　者：曲守权[1] 王鹤[1] 朱悦[1] 范广宇[1] 

机构地区：[1]宁波第六医院,浙江宁波315040

出　　处：《中国骨伤》2012年第2期143-146,共4页China Journal of Orthopaedics and Traumatology

摘　　要：目的:利用骨收获室内无应力的物理特性,观察无应力时骨重塑是否有方向性。方法:采用骨收获室模型的动物实验方法,5只日本大耳白雌兔。研究中不限制兔的活动。骨收获室是带有1个宽1mm的骨长入孔道的钛制圆柱形植入体,允许孔道内的成分被反复取出,仅伴有少量的对周围骨的干扰。先将骨收获室置放兔胫骨内8周,使其与骨达到较好的整合。8周后开始正式实验,其后每隔1个月收获组织。收获的组织,经固定、脱钙,采用石蜡包埋的组织切片。动物实施4阶段的4次手术:第1次空置;第2、3次收获的组织做纵向切片;第4次做横向切片。方向性分析:以每张切片所有骨细胞核方向的标准差为统计量,分析纵切面与横切面细胞的方向是否有差异。结果:收获室内长入组织,纵切面的细胞排列的方向性明显强于横切面,差异有统计学意义。结论:在无应力情况下,骨重建具有方向性。应力不是骨方向改建的直接指导因素。推测骨重建的方向性与骨收获室的结构特点相关,生理的方向性骨重建与应力的关系可能是通过血管介导,应力可能通过影响血管的方向性分布起作用。Objective：To study orientation remodeling without stress in bone harvest chamber. Methods：The bone harvest chamber （BHC） methodology is adopted in this study. Five female Japanese white rabbits were allowed unrestricted activity. The bone harvest chamber was a cylindrical Ti implant body with a transverse 1 mm wide canal for bone ingrowths. Retrieval of the contents of the canal was allowed with minimal disturbance to the surrounding bone or outer cylinder. After bone harvest chambers were implanted into the tibia of rabbits for 8 weeks,the chambers were considered to be osseointegrated with the bone. After harvested,the tissue were fixed and decalcified,then embedded in paraffin. Each rabbit was put into surgical operation 4 times for 4 stages：vacant for the first time;the tissue were cut into longitudinal sections at the second and third stages;harvesting tissues were cut into transverse sections at the fourth stage. Directional analysis：the standard deviation of the orientation of cell nucleus in each section was used as statistics,the difference between longitudinal section and transverse section were analyzed. Results：Of the tissue into bone harvest chamber,directionality of cells arranged was more significantly on longitudinal section than on transverse section,and there was statistical ignificecne. Conclusion：Under the no-stress circumstance of BHC,bone remodeling showed directivity. Stress is not the direct leading signal about bone reconstitution. The structure of BHC might be related to orientation remodeling,which suggests that the relationship between orientation and stress is mediated by blood vessel. The effect of stress may be to affect vessel distributing in some orietation.

关 键 词：骨重建 骨细胞 应力 物理 胫骨 

分 类 号：R687[医药卫生—骨科学]