融合蛋白dot-ELISA法检测猪囊尾蚴病人循环抗体  

A dot-ELISA test using fusion protein antigen for detecting cysticercosis antibody

作　　者：王敏[1] 舒晶[1] 徐之杰[1] 王光岳[1] 

机构地区：[1]哈尔滨医科大学寄生虫学教研室,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2000年第1期8-9,共2页Journal of Harbin Medical University

摘　　要：目的　检测用基因重组技术所获得的融合蛋白的特异性与敏感性。方法　用猪囊尾蚴cDNA文库筛选的含编码 2 8、18、14、3 4KDa抗原的基因片段阳性克隆 ,等比联合使用 ,以液相培养法经IPTG诱导重组噬菌体gt11 E .ColiY10 90表达融合蛋白 ,并用做抗原 ,用dot ELISA法诊断囊虫病。结果　血清以 1∶5 0倍稀释 ,囊虫病人血清 3份 ,阳性率为 83 3 % ,正常人和肝吸虫病人血清 3 0份 ,包虫病人血清 16份均为阴性。结论　与部分提纯囊虫抗原酶联免疫检测试剂盒相比 ,具有特异性强 。Objective To examine the specificity and sensitivity of the fusion protein produced by gene recombination technique.Methods The fusion protein antigens were obtained from the expression of the clones which encode 28,18,14 and 34 KDa fragments.Such clones were screened from cysticercus cellulosae cDNA library and induced into λ gt11/E.ColiY1090.Through fluid culture,the recombination espressed fusion protein which were detected by dot ELISA to diagnose cysticercosis.Results The results showed that the positive rate of 30 cases of cysticercosis was 83.3% with the serum dilution titer was 1∶50.While none of the samples from 30 healthy controls,30 cases of clonorchiasis and 16 cases of echinococcosis was positive.Conclusion The dot ELISA using fusion protein is higher specific than the ELISA dit made by practical purification antigen.And the assay is very convenient and easy to repeat.

关 键 词：融合蛋白 囊尾蚴病 DOT-ELISA 循环抗体 

分 类 号：R532.3[医药卫生—内科学]