顶空气相色谱法测定3种中药提取物中有机溶剂残留量  

Determination of organic residues in three kinds of herbal extracts by capillary gas chromatography with headspace sampling

作　　者：刘婷婷[1] 周伟 方进波[1] 叶茂[1] 丁茜[1] 叶小春[1] 陈家春[1] 

机构地区：[1]华中科技大学同济药学院天然药物化学与资源评价湖北省重点实验室,湖北武汉430030 [2]湖北省药品检验所,湖北武汉430064

出　　处：《中国医院药学杂志》2012年第4期290-292,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的:建立金刚藤、黄芪、延胡索3种中药提取物中正己烷、苯、甲苯、二甲苯、苯乙烯、二乙烯基苯有机溶剂残留量的检测方法。方法:采用毛细管气相色谱法配以顶空进样,以DB-5(柱长30 m,内径0.25 mm,涂布厚度0.25μm);毛细管柱进行分离,氢火焰离子化检测器检测。结果:6种大孔吸附树脂残留溶剂在30 min内能很好地分离,该方法线性良好,r在0.990 4～0.999 9之间;稳定性、回收率RSD值均小于7%;平均加样回收率在85.0%～110.3%之间。结论:该方法操作简单,结果准确可靠,能对金刚藤、黄芪、延胡索提取物中AB-8型大孔吸附树脂有机溶剂残留量进行检测控制。OBJECTIVE To establish a method for the determination o{ organic solvent residues including n-hexane, benzene, toluene, xylene, styrene, divinylbenzene in three kinds of extracts of Rhizoma Smilacis Chinae, Radix Astragali and Rhizoma Co- rydalis. METHODS The residues from AB- 8 macroporous adsorption resin were analyzed by headspace gas chromatography using DB- 5（30 m× 0. 25mm,0. 25 μm） capillary column and a FIE）. RESULTS All the 6 organic solvents were well separated in 30min. There was a good linearity relationship between peak area and concentration（r： 0. 990 4- 0. 999 9）. The RSDs of sta- bility and recovery were all less than 7 %. The average recovery rate was in the range of 85.0 % - 110. 3 %. CONCLUSION The method is simple,accurate and can be used for quality control. KEY WORDS：macroporous adsorption resin; organic solvent residues; headspace GC; Rhizoma Smilacis Chinae; Radix Astra- gall; Rhizoma Corydalis

关 键 词：大孔吸附树脂 有机溶剂残留量 顶空气相色谱 金刚藤 黄芪 延胡索 

分 类 号：R927[医药卫生—药学]