脂血和溶血对HBV DNA实时荧光定量PCR检测的影响  

Influence of hemolysis and lipoidemia on HBV DNA real-time PCR

作　　者：张翠莉[1] 刘萍[2] 魏文波[1] 

机构地区：[1]武警四川总队医院检验科,乐山61000 [2]武警四川总队成都分院检验科,610041

出　　处：《武警医学》2012年第2期150-152,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨溶血和脂血两种因素对HBV DNA实时荧光定量PCR方法的影响。方法 (1)收集临床HBV DNA标本,按HBV DNA浓度分为Ⅰ水平、Ⅱ水平;收集HBV DNA阴性的重度乳糜状脂血标本和正常人标本。将以上标本按不同比例混合,制作成极高TG浓度不同HBV DNA水平:AⅠ、AⅡ组;高TG浓度不同HBV DNA水平:BⅠ、BⅡ组;正常TG浓度不同HBV DNA水平:CⅠ、CⅡ组。实时荧光定量PCR扩增。(2)收集临床HBV DNA标本,每人采集3份血标本,其中2份标本进行-20℃冷冻不同时间,制成重度溶血DⅠ、DⅡ组;中度溶血EⅠ、EⅡ组;无溶血对照组FⅠ、FⅡ组。实时荧光定量PCR扩增。(3)利用方差分析各检测结果之间是否有统计学差异。结果不同浓度脂血和溶血,对两种水平HBV DNA荧光定量PCR检测结果均无统计学差异。结论溶血和脂血对实时荧光定量PCR检测HBV DNA结果无影响。Objective To study the influence of hemolysis and lipoidemia on real-time fluorescence quantitative polymerase chain reaction（RT-PCR） of HBV DNA.Methods（1）Clinical HBV DNA specimens were collected,and were divided into level I and level II according to HBV DNA concentrations.HBV DNA negative but severe blood lipid samples and normal blood samples were collected.These specimens were mixed in different proportions and divided into group A,B,C by different TG concentrations.（2）Clinical HBV DNA specimens were collected,three tubes of blood were obtained from each person,and two of them were frozen in a-20 ℃ refrigerator for different lengths of time to make hemolytic samples of different degrees.Then,they were divided into group D,E,F according to the degree of hemolysis.Results There is no statistically significant difference between the lipoidemia and hemolysis groups in the measurement of HBV DNA RT-PCR（P0.05）.Conclusions Lipoidemia and hemolysis do not affect the results of HBV DNA RT-PCR.

关 键 词：脂血 溶血 实时荧光定量PCR HBV DNA 

分 类 号：R392.11[医药卫生—免疫学]