重症急性胰腺炎伴胃肠动力障碍大鼠结肠黏膜下血管活性肠肽神经元的变化  

Changes of vasoactive intestinal peptide neurons in colonic submucous plexus of mice with severe acute pancreatitis complicated by gastrointestinal dysmotility

作　　者：程晓雯[1] 林中[1] 郑清华[1] 李小玲[1] 

机构地区：[1]桂林医学院附属医院消化内科,广西桂林541001

出　　处：《南京医科大学学报（自然科学版）》2012年第2期183-187,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：广西壮族自治区卫生厅中医药科技专项基金资助(GZKZ10-125)

摘　　要：目的:探讨结肠黏膜下神经丛血管活性肠肽(vasoactive intestinal peptide,VIP)神经元对重症急性胰腺炎伴胃肠动力障碍疾病的影响。方法:用逆行胰胆管注射牛磺胆酸钠的方法建立重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠模型,检测胰腺病理评分以及小肠推进比。利用全层铺片方法制作结肠黏膜下神经丛标本,双重免疫荧光法计数VIP阳性神经元占总神经元的百分比。结果:与正常大鼠比较,SAP组大鼠开腹后见大量腹水及腹壁、胰周皂化斑点,肠管扩张明显,胰腺病理评分明显增高(P<0.01),小肠推进比明显下降(P<0.01),VIP阳性神经元与总神经元百分比明显增高(P<0.01)。结论:重症急性胰腺炎大鼠胃肠动力障碍可能与结肠黏膜下神经丛VIP神经元增多有关。Objective： To investigate potential effects of vasoactive intestinal peptide （VIP） in colonic submucous plexus of mice with severe acute pancreatitis（SAP） complicated by gastrointestinal dysmotility. Methods： Twenty Sprague Dawley rats weighing 200-250 g were distributed into two groups： induced acute pancreatitis and control group. Acute pancreatitis was induced by intra- ductal infusion of 5% sodium taurodeoxycholate,and small intestinal impelling ratio were detected. Pancreatic lesion was scored. Whole mount samples of submucous plexus were prepared and stained with double immunofluorescence to observe the morphology of VIP neurons. The percentage of VIP neurons in the total neurons was calculated. Results： Compared with the control gourp, the small intestinal impelling ratio in the SAP group was significantly lower （P 〈 0.01 ）,the score of pancreatic lesion was remarkably higher（P 〈 0.01 ）,the VIP neurons were bigger and more deeply stained,and the nerve fibers between ganglions of the VIP were thicker,and the percentage of VIP neurons was significantly higher （P 〈 0.01 ）. Conclusion： A high expression level of VIP neurons in colonic submucous plexus may be one of the nervous mechanisms underlying gastrointestinal dysmotility in SAP rats.

关 键 词：重症急性胰腺炎 胃肠动力 功能障碍 血管活性肠肽 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学] R576[医药卫生—基础医学]