皮肤型红斑狼疮皮损中鳞状细胞癌抗原1的表达和意义  

The expression and significance of squamous cell carcinoma antigen-1 in cutaneous lupus erythematosus

作　　者：方圣[1] 单葵[1] 周汛[1] 陈爱军[1] 李惠[1] 

机构地区：[1]重庆医科大学附属第一医院皮肤科,重庆400016

出　　处：《南京医科大学学报（自然科学版）》2012年第2期204-207,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：重庆市自然科学基金(cstc2011JJA10039)

摘　　要：目的:研究鳞状细胞癌抗原1(squamous cell carcinoma antigen-1,SSCA1)在皮肤型红斑狼疮(cutaneous lupus erythe-matosus,CLE)皮损中的表达和意义。方法:培养原代角质形成细胞,提取蛋白样品采用固相pH梯度双向凝胶电泳进行分离,应用ImageMaster 2D Platinum 5.0软件对图像进行匹配分析,选取差异表达个蛋白质点,经基质辅助激光解吸附飞行时间质谱进行质谱鉴定。并通过免疫印迹验证其表达水平。结果:体外培养角质形成细胞成功,获得重复性较好的双向电泳图谱,和正常皮肤相比,SCCA1在CLE皮损角质形成细胞中表达升高,免疫印迹验证与双向电泳结果一致。结论:SCCA1在CLE皮损角质形成细胞中表达升高,可能与CLE的发生发展有关,但SCCA1表达升高的具体机制仍需进一步研究。Objective： To study the expression and significance of squamous ceil carcinoma antigen-1 （SCCA1） in keratinocytes from the lesion of cutaneous lupus erythematosus. Methods： The keratinocytes were isolated from the human skin and cultured in serum-free media. The total proteins of keratinocytes were separated by 2-dimensional gel electrophoresis. The differential proteins were analyzed using ImageMaster 2D analysis software after staining and identified by MALDI-TOF-MS. The expressional levels of the proteins were further confirmed by Western blot analysis. Results： We successfully in vitro cultured human skin primary ker- atinocytes and established 2-dimensional electrophoresis profiles with high resolution and reproducibility. The SCCA1 protein was up- regulated in the keratinocytes from lesion of CLE compared to the normal group, and the result from both proteomics approach agreed with that of Western blot analysis. Conclusion： The SCCA1 protein was up-regulated in the keratinocytes from lesion of CLE,which may be related to the development of CLE. However,the pathogenesis of over expression of SCCA1 need further study.

关 键 词：皮肤型 红斑狼疮 鳞状细胞癌抗原1 角质形成细胞 蛋白组 

分 类 号：R593.24[医药卫生—内科学]