磁共振动态磷谱技术评估2型糖尿病患者子女的骨骼肌线粒体功能  

Evaluation of mitochondrial function of skeletal muscle in type 2 diabetic patients offspring using dynamic ^(31)P magnetic resonance spectroscopy

作　　者：涂慧娟[1] 吴飞云[1] 秦斌[1] 陈婷[1] 齐静[1] 

机构地区：[1]南京医科大学第一附属医院放射科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2012年第2期249-254,共6页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:评价磁共振动态磷谱技术对评估2型糖尿病患者子女骨骼肌能量代谢和线粒体功能的价值。方法:对21名2型糖尿病患者子女和18名健康志愿者的股四头肌进行磁共振动态磷谱检查,获取静息、运动、恢复3个状态的波谱数据。对无机磷(Pi)、磷酸肌酸(PCr)、三磷酸腺苷(ATP)等化合物的峰下面积进行定量分析,通过生化反应平衡公式计算二磷酸腺苷(ADP)和细胞内pH值,并利用指数曲线拟合分析Pi、PCr和ADP的时间常数和恢复速率。结果:糖尿病患者子女组及正常对照组静息期Pi、PCr、ADP、β-ATP的含量及Pi/PCr无统计学差异。运动末期糖尿病患者子女组β-ATP低于正常对照组。恢复期糖尿病患者子女组多帧波谱PCr、β-ATP、pH均低于正常对照组,第3帧Pi/PCr比值比对照组高。恢复期糖尿病患者子女组PCr恢复速率明显低于正常对照组PCr恢复速率。结论:磁共振动态磷谱技术可以无创性检测2型糖尿病患者子女的能量代谢受损及线粒体功能状态。Objective： To evaluate the energy metabolism and mitochondrial function of skeletal muscle in type 2 diabetic patients offspring using dynamic 31^p magnetic resonance spectroscopy（31^P-MRS）. Methods： Dynamic 31^p-MRS was performed on 21 type 2 diabetes patients offsprings and 18 healthy volunteers. Spectra were acquired from quadriceps muscle while subjects were at rest,exercise and recovery. The peak area of inorganic phosphate （Pi）,phosphocreatine （PCr） and adenosine triphosphate （ATP） were measured. The concentration of adenosine diphosphate （ADP） and intracellular pH value were calculated from the biochemistry reaction equilibrium. The time constant and recovery rate of PCr,Pi and ADP were analyzed using exponential curve fit. Results： There were no significance differences of Pi, PCr,ADP, β-ATP and the ratio of Pi/PCr between the type 2 diabetes patients offsprings and the healthy volunteers at rest. The type 2 diabetes patients offsprings showed significantly lower β-ATP concentration at the end of exercise compared to the healthy subjects. At recovery phase,the PCr and β-ATP concentration and pH of the type 2 diabetes patients offsprings were lower than those of the healthy subjects. The ratio of Pi/PCr of the type 2 diabetes patients offspring is significantly higher than that of the healthy subjects at the third spectrum of recovery phase. The mean PCr recovery rate of the offspring is significantly slower than that of the healthy subjects. Conclusion： Noninvasive 31^P-MRS could detect the energy metabolism inefficiency and mitochondrial function impairment in skeletal muscle of type 2 diabetes patients offsprings.

关 键 词：2型糖尿病患者子女 磁共振磷谱 骨骼肌 线粒体功能 

分 类 号：R685[医药卫生—骨科学] R445.2[医药卫生—外科学]