FMR1基因敲除对雄性小鼠生殖功能的影响  

The influence of FMR1 gene knockout on the reproduction of male mice

作　　者：祝亚桥[1] 周兴[1] 陈盛强[2] 

机构地区：[1]广州医学院第二附属医院泌尿外科,510260 [2]广州医学院第二附属医院神经科学研究所,510260

出　　处：《实用医学杂志》2012年第4期564-567,共4页The Journal of Practical Medicine

摘　　要：目的:利用脆性X智力低下1号基因(fragile xmental retardation-1,FMR1)敲除鼠,研究FMR1基因对雄性小鼠精子生成的调节及生殖功能的影响。方法:将雄性FMR1基因敲除鼠(KO)和野生型FVB小鼠(WT)分别与野生型FVB成年母鼠合笼,观察母鼠怀孕率,母鼠产仔数及能生育的雄鼠数量;测定雄鼠血清TTFSH及LH浓度;收集睾丸、附睾组织,对左附睾尾部精子进行计数、活率、形态等分析;右侧常规石蜡包埋,切片、HE染色。结果:KO组母鼠受孕率41.7%低于WT组87.5%(P<0.05),能够生育的KO雄鼠比率41.7%也低于WT组91.7%(P<0.05),与KO组和WT组小鼠交配的雌鼠平均产仔数分别为6.50±2.27和8.22±3.03(P>0.05),两组小鼠血清T,FSH,LH浓度无统计学差异。KO组的睾丸附睾病理切片与WT组比较未见明显异常,其精子活率及各种畸形率与WT小鼠比较均没有统计学差异(P>0.05)。结论:可以推测FMR1基因对雄性生殖系统发育有一定的影响,Fmr1基因的缺失降低了雄性小鼠生育率,但对精子生成、畸形率等未见明显影响,其对雄性生殖系统影响机制还有待进一步的实验研究。Objective To investigate the influence of fragile x mental retardation-1（FMR1） gene on the spermatogenesis and reproduction of male mice.Methods FMR1 knockout（KO）male mice and wild type（WT）male mice were mated with wt female mice.The number of litters,pregnancy rate and male mice having offsprings were counted.Serum T,FSH and LH concentrations were also measured.The density,mortality and morphology of the left cauda epididymis sperms were analyzed,HE staining was performed on the right side.Results The pregnance rate of wt female mice mated with FMR1KO males was significantly lower than the control group（41.7% vs.87.5%,P 0.05）,the average litter size was 6.50 ± 2.27 and 8.22 ± 3.03（P 0.05）.Male fertility showed that 41.7% of KO mice had pups,whereas 91.7% of the mice had pups in the control group（P 0.05）.No obvious pathological difference in testes and epididymides was found between the two groups.There was no significant difference in the sperm count,motility and abnormal sperm ratio in two groups.Conclusions FMR1 gene may play an important role in the development of male genital system and the absence of FMR1 reduce the reproduction of male mice.The mechanisms of FMR1 gene influencing the male genital system are needed to further study.

关 键 词：脆性X智力低下蛋白质 基因敲除 生殖 精子发生 

分 类 号：R697[医药卫生—泌尿科学]