2009-2010年河南省小麦白粉菌群体毒性及其遗传多样性分析  

Virulence and genetic diversity analyses of wheat powdery mildew population in Henan Province during 2009-2010

作　　者：李亚红[1,2] 曹丽华[1] 周益林[2] 宋玉立[3] 何文兰[3] 段霞瑜[2] 杨共强[3] 

机构地区：[1]河南农业大学植物保护学院,郑州450002 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [3]河南省农业科学院植物保护研究所,郑州450002

出　　处：《植物保护学报》2012年第1期31-38,共8页Journal of Plant Protection

基　　金：国家重点基础研究发展计划课题(2011CB100403,2006CB100203);“十一五”科技支撑计划(2006BAD08A05);公益性行业(农业)科研专项(3-15)

摘　　要：利用40个鉴别寄主对河南省5个小麦产区的44个单孢子堆纯化分离的小麦白粉病菌进行毒性鉴定分析,并采用多基因家系法进行了遗传多样性分析。结果显示,供试群体对Pm8、Pm1、Pm3a、Pm3b、Pm3c、Pm3f、Pm3e、Pm5、Pm6、Pm7、Pm17、Pm19、Pm34等抗病基因的毒性频率分别达到90%以上,已不能再作为抗源利用;对Pm12、Pm16、Pm21、Pm18、Pm2、Pm2+6、Pm2+MLD、Pm5(Mli)、Pm23、Pm30、Pm4a等基因的毒性频率低于15%,仍可以在抗病育种中加以利用;河南省的小麦白粉病菌存在地理之间的差异,同时病菌在地区之间有自西南向东北/东方向的传播。表明省内群体中存在着丰富的遗传多样性。In order to disclose the genetic structure and the genetic relationship of wheat powdery mildew population in Henan Province,the virulence of 44 wheat powdery mildew isolates collected from 5 wheat growing areas in Henan was analyzed by using 40 differential hosts and multigene genealogy.The result of virulence survey reveals that the virulence frequencies to the resistance genes Pm8,Pm1,Pm3a,Pm3b,Pm3c,Pm3f,Pm3e,Pm5,Pm6,Pm7,Pm17,Pm19,and Pm34 have reached 90% or higher,respectively,indicating that these resistant genes cannot be used as mildew resistance sources any more.The virulence frequencies of the mildew population tested to resistance genes Pm12,Pm16,Pm21,Pm18,Pm2,Pm2＋6,Pm2＋MLD,Pm5（Mli）,Pm23,Pm30,and Pm4a were lower than 15%,and these genes can still be used in breeding for mildew resistance.There is differentiation among geographical areas in wheat powdery mildew populations in Henan Province and the pathogen disperse between the areas studied.The direction of dispersal is from the southwest to the northeast/east.The genetic diversity is relatively rich.

关 键 词：小麦白粉菌 毒性鉴定 多基因家系 遗传多样性 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]