灰霾天气细颗粒物对人支气管上皮细胞凋亡的影响  

Effects of Haze Fine Particulate Matter on Apoptosis of Human Bronchial Epithelial Cells

作　　者：覃辉艳[1] 彭晓武[2] 蒙智娟[1] 黄炯丽[1] 李琴[1] 韦小敏[1] 邹云锋[1] 

机构地区：[1]广西医科大学公共卫生学院,广西南宁530021 [2]环境保护部华南环境科学研究所,广东广州510655

出　　处：《环境与职业医学》2012年第2期86-88,共3页Journal of Environmental and Occupational Medicine

基　　金：2007年国家环保公益性行业科研专项项目(编号:200709004)

摘　　要：[目的]探讨灰霾天气细颗粒物(PM2.5)对人支气管上皮细胞(human bronchial epithelial cells,16-HBE)凋亡的影响。[方法]分别以8、16、32、64、128μg/mL浓度的PM2.5作用于16-HBE 24、48、72h,检测PM2.5对16-HBE细胞存活率及凋亡率的影响。[结果]染毒24、48、72h后,64、128μg/mL组细胞存活率均明显低于对照组(P<0.05),细胞凋亡率均明显高于对照组(P<0.05);72h各浓度组细胞存活率均低于24h相应处理组(P<0.05),当染毒浓度大于8μg/mL时,72h各浓度组细胞总凋亡率与24h相比均明显增加(P<0.05)。[结论]灰霾天气细颗粒物PM2.5对16-HBE具有细胞毒性,能诱导其凋亡,且具有剂量和时间反应关系。[Objective] To explore the effect of haze particulate matter PM2.5 on apoptosis of human bronchial epithelial cells（16-HBE）.[Methods] Groups of cultured 16-HBE cells were exposed to 8,16,32,64 and 128 μg/mL PM2.5 respectively for 24,48 and 72 h of incubation to detect the cell survival and apoptosis rates.[Results] After 24,48 and 72 h of interference,compared with the negative control group,the cell survival rates of 64 and 128 μg/mL groups decreased significantly（P 0.05）,and the cell total apoptosis rates increased significantly（P 0.05）.Compared with the corresponding 24 h groups,the cell survival rates of 72 h groups decreased significantly（P 0.05）,and the apoptosis rates increased significantly except when treated with 8 μg/mL（P 0.05）.[Conclusion] Haze particulate matter PM2.5 possess cytotoxic effect on 16-HBE to induce apoptosis in a dose-and time-dependent manner.

关 键 词：灰霾天气 细颗粒物 人支气管上皮细胞 细胞凋亡 

分 类 号：R322.34[医药卫生—人体解剖和组织胚胎学]