七氟醚预处理大鼠肾缺血-再灌注损伤中HSP70的表达及保护机制  

Expression of HSP70 and protective mechanism in the sevoflurane preconditioning of renal damages caused by ischemic reperfusion

作　　者：顾福萍[1] 沈洪[1] 李睿明[1] 徐伯赢[1] 顾红光[1] 

机构地区：[1]浙江省湖州师范学院医学院,浙江湖州313000

出　　处：《中国急救医学》2012年第2期148-152,共5页Chinese Journal of Critical Care Medicine

基　　金：浙江省湖州市自然科学基金资助项目（No.2009YZ05）

摘　　要：目的 观察七氟醚预处理大鼠肾缺血-再灌注损伤模型中HSP70的表达变化,探讨其保护作用机制.方法 72只SD雄性大鼠随机分为对照组（C组）、缺血-再灌注组（I/R组）、七氟醚预处理组（S组）,每组各三个时相（4、12、24 h）.所有大鼠于实验结束时测定肾组织过氧化物歧化酶（SOD）活性、丙二醛（MDA）水平及血清肌酐（Cr）、尿素氮（BUN）水平.观察肾组织病理变化,免疫组化法检测大鼠肾组织热休克蛋白70（HSP70）、核转录因子-κB（NF-κB）的表达情况.结果.与C组比较,I/R组和S组HSP70表达均显著增加（P〈0.05）;与I/R组比较,S组HSP70表达在12 h达到高峰,并且显著高于I/R组;C组NF-κB几乎不表达,I/R组NF-κB表达显著增加（P〈0.05）,并且随再灌注时间的延长而增加;S组再灌注4、12、24 h NF-κB表达显著低于I/R组（P〈0.05）.与C组比较,I/R组和S组MDA显著升高而SOD显著降低（P〈0.05）;与I/R组比较,S组SOD显著升高而MDA显著降低（P〈0.05）;与C组比较,随再灌注时间的延长,I/R组和S组BUN、Cr均显著升高;与I/R组比较,S组12、24 h BUN、Cr均显著低于I/R组;S组肾损伤的病理变化较I/R组显著减轻.结论.七氟醚预处理能诱导肾缺血-再灌注大鼠模型HSP70的表达增强和提早表达,减弱NF-κB活化,增加肾缺血-再灌注后氧自由基的清除能力,实现对肾缺血-再灌注损伤的保护作用.Objective To explore the effect and protective mechanism of sevoflurane preconditioning by observing the expression Of the HSP70, NF - KB in rat model of renal ischemia - reperfusion injury（l/R）. Methods 72 SD male rats were randomly divided into control group （C group） , ischemia -reperfusion group （ l/R group） , sevoflurane preconditioning group （S group） ,Each divided into 4 h, 12,24 h 3 subgroups. End of the experiment all rats were measured SOD activity, MDA levels in renal tissue, and serum creatinine （ Cr）, blood urea nitrogen（BUN） levels. Renel pathological changes observed by optical microscopy and detected expression of HSP70, NF - KB in lena] tissue by immunohistochemistry. Results Compared with the C group, the level of HSP70 in renal tissue were significantly increased in l/R group and S group （ P 〈 0.05 ） ; the level of HSP70 in S group while HSP70 expression peaked at 12 h were higher than l/R group which indicated the expression of HSP70 in advance and enhanced;Immunohistochemical results revealed that expression of NF - KB in renal tissue were significantly higher in l/R group and S group than those in C group,but the expression of NF -KB in renal tissue were significantly lower in S group than those in l/R group in 4 h, 12 h,24 h after ischemic reperfusion. Compared with the C group, MDA were increased significantlyand SOD were significantly reduced in VR group and S group. SOD were reduced in S group; Compared with Compared with IZR group, MDA were increased and the C group, the level of BUN, Cr in serum were significantly increased in the I/R group and S group; the level of BUN, Cr of S group were significantly lower than the I/R group. In comparison to I/R group, renel pathological changes in S group were alleviated significamly. Conclusion Sevoflurane preconditioning can reduce rat renal ischemia- reperfusion injury by enhancing the expression of HSP70, Reducing NF - KB activation and Increasing oxygen free radical scavenging after rat renal ischemia -reperfusio

关 键 词：七氟醚 预处理 热休克蛋白70(HSP70) 核转录因子-κB(NF-κB)缺血-再灌注损伤 肾脏 

分 类 号：R692[医药卫生—泌尿科学]