菜用大豆高效胚尖离体再生基因型筛选  

Selection of High-efficient Regeneration Genotype from Embryonic Tips of Vegetable-type Soybean

作　　者：钟灿[1] 肖深根[1] 朱保葛[3] 薛仁镐[4] 卫志明[2] 朱木兰[2] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]中国科学院上海生命科学研究院,植物生理生态研究所,国家植物基因研究中心(上海),上海200032 [3]中国科学院遗传与发育生物学研究所,北京100101 [4]青岛农业大学生命科学学院,山东青岛266109

出　　处：《大豆科学》2012年第1期9-12,共4页Soybean Science

基　　金：转基因生物新品种培育重大专项资助项目(2009ZX08010-009B,2009ZX08004-009B)

摘　　要：以华东地区4个主栽菜用大豆品种(交大05-133、交大02-89、沪宁96-10、青酥二号)的胚尖为起始外植体,研究消毒方法、预培养天数、6-BA浓度和培养基组合等对不定芽的诱导和伸长的影响。结果表明:用0.1%HgCl2消毒10 min后配合5%的NaClO消毒5 min,消毒效果最佳,胚尖活力好,且适用于各个品种;预培养时间为2 d,6-BA浓度为3.0 mg.L-1,有利于菜用大豆不定芽的诱导;1.0 mg.L-16-BA+0.1 mg.L-1NAA培养基组合有利于增加有效不定芽数;0.05 mg.L-16-BA+0.1 mg.L-1IBA培养基组合有利于不定芽的伸长;交大05-133为最佳胚尖离体再生基因型,其分化频率为90.86%,诱导15 d后外植体平均不定芽数为4.65个,不定芽平均长度为1.38 cm。To study the effect of sterilization,pre-culture days,different concentrations of 6-BA and medium formulations on induction and elongation of adventitious buds,embryonic tips from four vegetable-type soybean varieties ＇Jiaoda 05-133＇,＇Jiaoda 02-89＇,＇Huning 96-10＇ and ＇Qingsuerhao＇ were used as the initial explants.The results showed that the optimal sterilization was using 0.1% HgCl2 for 10 min and 5% NaClO for 5 min.The optimal adventitious buds induction formulation was MSB5 base medium with 3.0 mg·L-1 6-BA,and the optimal pre-culture days of embryonic tips was 2 d.The combination of 1.0 mg·L-1 6-BA and 0.1 mg·L-1 NAA benefited to produce many adventitious buds.The combination of 0.05 mg·L-1 6-BA and 0.1 mg·L-1 IBA benefited to the elongation of adventitious buds.Among the four varieties,the embryonic tips from Jiaoda 05-133 is the best one for regeneration,with 90.86% of adventitious buds induction rate,4.65 of mean number of adventitious buds and 1.38 cm of mean length of adventitious buds after culture for 15 d.

关 键 词：菜用大豆 胚尖 基因型筛选 离体再生 

分 类 号：S565.1[农业科学—作物学]