反相高效液相色谱法选择性测定血浆中普罗帕酮对映体  

Selective Determination of Propafenone Enantiomers Level in Human Plasma by Reversed-Phase Liquid Chromatography

作　　者：陈鹰[1] 裘军[2] 吴梅 张宜[1] 李高[2] 汤韧[1] 

机构地区：[1]广州军区武汉总医院药局,430070 [2]同济医科大学药学院

出　　处：《中国药师》2000年第1期36-37,共2页China Pharmacist

摘　　要：采用GITC柱前衍生化法同时分离和测定人血浆中(R)-PPF和(S)-PPF的浓度,在50～500ng·ml^(-1)浓度范围内有良好的线性关系;血浆中(S)-PPF的提取回收率、相对回收率、日内RSD、日间RSD分别为88.76％～91.45％、98.76％～101.22％、5.62％～6.79％、7.65％～9.01％;(R)-PPF的分别为88.98％～91.33％、98.31％～100.56％、5.02％～7.03％、7.88％～9.05％。A reversed-phase liquid chromatqgraphy analysis with pre-coloum derivatization for determining the level of Propafenone enantiomers in human plasma was established. The standard curves were linear in the range of 50-500 ng/ml. The refined-recovery rate, relative recovery rate, within-day RSD and between-day RSD for (S)-PPF were 88.76 %-91.45 % , 98.76 %- 101.22 % , 5.62 %-6.79 % , and 7.65 %-9.01 % , respectively. Those for (R)-PPF were 88.98 % -91.33 %, 98.31 %-100.56 %, 5.02 %-7.03 %, and 7.88 %-9.05 %, respectively.

关 键 词：普罗帕酮 对映体 血药浓度 HPLC 反相 

分 类 号：R972.2[医药卫生—药品] R969.1[医药卫生—药学]