建立小鼠原位肝移植模型的体会  

Establishment of orthotopic liver transplantation model in mice

作　　者：程琪[1] 高丹[1] 龙新[1] 朱鹏[1] 陈孝平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肝脏外科中心 ,武汉430030

出　　处：《腹部外科》2012年第1期47-49,共3页Journal of Abdominal Surgery

摘　　要：目的探讨小鼠原位肝移植模型的技术改进。方法按“双袖套”法完成小鼠原位肝移植模型50台，对供者手术、袖套安装及受者肝上下腔静脉吻合等方法进行技术改进，总结手术技巧，并评估手术时间、手术成功率及长期存活率。结果供者手术时间、受者无肝期时间及受者手术时间分别为（18±2）min、（17±2）min、（43±3）min。手术成功率为92％，100d存活率为85％。结论该方法可快速获取高质量供肝，同时无肝期的缩短可保证较高的手术成功率。Objective To investigate the technique modification of orthotopic liver transplanta tion （OLT） model establishment in mice. Methods According to ＂two-cuff＂ method, 50 OLT models in mice was established. The modifications included the surgical procedures for donors, fixation of the cuff, and the anastomosis of superior and inferior caval vein. Results The operating time of harvesting donor＇s liver,anhepatic phase and recipients were （ 18 ± 2）, （17 ± 2） and （43 ± 3） rain, respectively. The model success rate was 92 %,and the 100-day survival rate was 85 %. Conclusion High quality liver could be harvested by this modified method, and short anhepatic phase ensured the high model success

关 键 词：小鼠 肝移植 模型 动物 

分 类 号：R657.3[医药卫生—外科学]