桂枝茯苓丸对S180荷瘤鼠移植性肿瘤细胞转移影响的实验研究  

Experimental study on inhibitory effect of GFW on transplantable tumor cell metastasis in S180 tumor-bearing mices

作　　者：罗晓庆[1] 孙济宇[2] 王琪[1] 官杰[1] 

机构地区：[1]齐齐哈尔医学院免疫教研室,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院附属第三医院,黑龙江齐齐哈尔161006

出　　处：《中国中药杂志》2012年第4期520-523,共4页China Journal of Chinese Materia Medica

基　　金：黑龙江省教育厅项目(11511458)

摘　　要：目的:探讨桂枝茯苓丸(GFW)对S180荷瘤小鼠肿瘤转移的影响。方法:复制S180荷瘤小鼠模型,分为模型组、桂枝茯苓丸组、环磷酰胺组、联合用药组。采用ELISA法检测各组小鼠血清中VEGF的含量,SABC免疫组化法检测肉瘤组织中转移抑制基因nm23H1表达及细胞黏附分子CD44表达。结果:与模型组相比,GFW能显著降低荷瘤鼠血清中VEGF的含量(P<0.05)。GFW可促进转移抑制基因nm23H1的表达,抑制细胞黏附分子CD44的表达,与模型组比较,差异有统计学意义(P<0.05)。结论:GFW具有一定的抑制肿瘤转移的作用。Objective:To detect the effect of GFW on tumor cell metastasis in S180 tumor-bearing mice.Method:S180 tumor-bearing mice model were replicated and divided randomly into 4 groups:the model group,the GFW group,the cyclophosphamide group and the combination administration group.VEGF in serum on each group was detected by ELISA,and the expression of metastasis suppressor gene nm23H1 and cell adhesion molecule CD44 in Sarcoma were detected by SABC immunohistochemical assay.Result:Compared with the model group,the GFW group showed a significant decrease in VEGF in serum(P<0.01).From their statistically significant difference,GFW was proved to promote the expression of metastasis suppressor gene nm23H1 and inhibit the expression of cell adhesion molecule CD44(P <0.05).Conclusion:GFW has an effect on inhibiting tumor metastasis to some extent.

关 键 词：桂枝茯苓丸 肿瘤转移 VEGF NM23H1 CD44 

分 类 号：R285.5[医药卫生—中药学]