UPLC法测定不同产地不同部位牡丹皮中6种活性成分  

UPLC determination of six active components in different parts of Moutan Cortex from different habitats

作　　者：范旭航[1] 马天成[1] 沈旭[1] 张文婷[2] 毕开顺[3] 贾英[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]浙江省食品药品检验所,浙江杭州310004 [3]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中成药》2012年第2期317-320,共4页Chinese Traditional Patent Medicine

基　　金："重大新药创制"科技重大专项(2009ZX09308-003;2009ZX09504-004)

摘　　要：目的建立同时测定产自安徽和山西省的牡丹皮皮层和髓部中没食子酸、芍药苷、苯甲酸、苯甲酰氧化芍药苷、苯甲酰芍药苷和丹皮酚的UPLC方法,为确立最佳的牡丹皮加工方法提供依据。方法牡丹皮甲醇液(超声提取)供HPLC分析采用ACQUITY UPLC,HSS T3色谱柱(2.1 mm×100 mm,1.8μm),流动相为乙腈-0.05%磷酸水溶液梯度洗脱,检测波长230 nm。结果没食子酸、芍药苷、苯甲酸、苯甲酰氧化芍药苷、苯甲酰芍药苷和丹皮酚分别在3.607～72.14、12.00～240.0、1.600～32.00、0.998～19.96、6.008～120.2、22.42～448.4μg/mL范围内呈良好的线性关系。平均回收率分别为98.3%,99.5%,101.2%,98.1%,100.3%,98.7%。不同产地牡丹皮中各成分变化规律基本一致。牡丹皮栓皮中各成分质量分数均较高,而木心中也含有一定的活性成分。结论牡丹皮加工过程中可以考虑不刮掉栓皮。整体质量连丹皮优于刮丹皮。AIM To determine the chemical constituents in cortex and pith of Moutan Cortex,including gallic acid,peoniflorin,benzoic acid benzoyloxypaeoniflorin,benzoylpaeoniflorin and peaonol,collected from Anhui and Shanxi provinces for quality control and improvement in processing.METHODS The fifteen batches of Moutan Cortex were determined on an HSS T3 column（2.1 mm×100 mm,1.8 μm） eluted with the mobile phase consisting of water containing 0.05% phosphoric acid and acetonitrile in gradient mode and the detection wavelength was set at 230 nm.RESULTS The standard curves of six active components showed a good linearity in 3.607-72.14 μg/mL,12.00-240.0 μg/mL,1.600-32.00 μg/mL,0.998-19.96 μg/mL,6.008-120.2 μg/mL,22.42-448.4 μg/mL,respectively.The average recoveries were 98.3%,99.5%,101.2%,98.1%,100.3%,98.7%,respectively.The change in six active components in Moutan Cortex from different habitats was roughly the same.It was demonstrated that a higher level of active constituents was found in cortex while a lower level was found in pith.CONCLUSION The contents of active constituents in Moutan Cortex with cutis tissue is higher than that without cutis tissue.It is recommended that the processing of Moutan Cortex may be considered to preserve the cutis tissue.

关 键 词：牡丹皮 活性成分 不同部位 UPLC 

分 类 号：R284.1[医药卫生—中药学]